Bio 1010 Cell Bio Flashcards
What are the 3 components of the cell theory?
(1) All organisms are composed of one or more cell(s)
(2) The cell is the structural unit of life
(3) Cells can only arise from existing cells
What is the difference between TEM and SEM Microscopes?
Both are electron microscopes, TEM is a transmission EM that examine the internal structure of a cell/specimen, wheres as an SEM (scanning EM) creates a 3D image of the specimen/cell by scanning the surface
What makes a prokaryotic cell differ from a eukaryotic cell?
Prokaryotes do not have membrane-bound organelles, and in fact instead of a nucleus have a “region” of their DNA known as the nucleoid (circular DNA).
Explain why cells cannot grow infinitley in size (why are they so small)?
They are small because of the necessary surface-to-volume ratio: as volume increases, SA begins to decrease, posing a problem as the membrane serves as a way for gas, food, and waste exchange.
What is the nuclear lamince?
The structure surrounding the nuclear envelope, providing stucture for the nucleus.
It disintegrates during mitosis.
What is the function of the nucleolus?
It is the smaller region found in the nucleus, that makes ribosomes (the small and large subunits).
What are the 3 places ribosomes can be found?
(1) In the cytosol (free ribosomes)
(2) Bound to membranes of the ER or nucleus
(3) Mitochondria (structurally different from first 2)
What are some of the functions of the smooth ER?
- Lipid synthesis
- Detoxifying the cell (i.e. a lot of smooth ER in the liver)
- Storing Ca Ions
What is the function(s) of the rough ER?
Primarily responsible for making proteins-to be on membrane, exported out of cell, or to be sent to other organelles.
Where do the proteins made in the ER go after? and how do they get there?
They are transported via vesicles, created in the lumen of the ER, to the Golgi Apparatus.
What are the compartments of the Golgi called? Explain the Cisternal Maturation Model.
The compartments of the Golgi are called cisternae, there are usually 7 of them. They start by collecting vesicles from the ER to form cistern #1 (this side is called the CIS cisternae). From there, the cistern begins moving down the line to the TRANS side, as the last cisternae becomes recycled (breaks apart into vesicles, carried to designated location).
Explain the function of the Golgi Apparatus
The Golgi modifies porteins sent in from the ER, by adding sugars, or trimming proteins, etc.
The Golgi residence enzymes are what aid in this.
How does the lysosome often form? What is its function?
The lysosome commonly comes from a vesicle that buds off from the trans side of the golgi.
The lysosome acts as a stomach of the cell, by breaking down polymers into their monomers for uses elsewhere in the cell. It can also break down entire organelles.
It does this via hydrolytic enzymes (recall polymers break down via hydrolytic reaction) in which only work in low pHs.
What is Autophagy?
Autophagy is the process of breaking down damaged or old cellular components, such as organelles or large proteins, so their monomers can be re-used.
This often happens via the lysosome.
What are the 3 main components of the cytoskeleton? List them in order of increasing size.
(1) Microfilaments
(2) Intermediate filaments
(3) Microtubules
Describe the structure of a microfilament, and what is it primarily composed of.
Microfilaments are rope-like structures that make up part of the cytoskeleton. They are made exclusively of a protein called actin (or g-actin, as it is a globular protein).
What protein do microfilaments often associate with? What is the function of their interaction?
They associate with the motor protein: Myosin.
This interaction has a few main functions:
(1) Muscle contraction/general contraction- the myosin can pull actin close, or keep it relaxed
(2) Cytokinesis via contraction- The microfilaments form a belt around the cell (in the extracellular matrix) and the myosin can pull on this belt, squeezing the cells to form into 2
(3) The microfilaments can act as a railway for myosin to walk on, myosin can attach the the microfilaments, as well as another structure (vesicles, organelles) to move them around the cell
Where are microfilaments typically found?
The extracellular matrix and the cortex.
Describe the structure and composition of microtubules.
Largest of the 3 cytoskeletal components, hollow-like rods.
Composed of a protein called Tubulin, existing only in dimers of a and b tubulin. The dimers form chains called protofilaments, and when there are 13 protofilaments they form a ring, which becomes the microtubule with the hollow interior.
Since the tubulin occurs as dimers, one end of the microtubule is the B (plus end) and one is the A (minus end). The plus end is where the microtubule can be assembled or de-assembled quickly.
Where do the microtubules come from in the cell? Where are they mainly located?
Microtubules grow out of the centrosomes, which is composed of centrioles, and when centrioles are in pairs they are at right angles to eachother.
Each centriole is made of 9 triplets of microtubules. They then act as railway tracks for the cell, or as extensions of the cell (flagella or cilia).
What motor proteins do microtubules interact with?
They interact with Kinesins (walks toward plus end), and Dynin (walks toward minus end).
Compare and contrast Flagella and Cilia.
Both are extensions off of a cell containing microtubules. They both allow cells to move, flagella are typically 1-2 per cell and have a snake-like motion. Cilia usually come in large number on cells, and have a rapid back-and-forth motion.
In some cases, cilia can occur by itself and in that case it is not for mobility, but rather a cell-signalling mechanism.
Explain the structure of the microtubules that make up a flagellum or cilia.
Arranged in a 9+2 arrangement: 9 doublet microtubules, and 2 in the centre holding the structural-ring together. This is called an axoneme, and extends from an area of the centriole known as the basal body.
Note nonmotile primary cilia have a 9+0 arrangement.
These
Explain the role the motor protein Dynein has on cellular extensions.
Dynein attached it self to 2 doublet pairs of the microtubules in the flagellum/cilium, and walk toward the minus end of he microtubule. This uses ATP and causes the bending motion of the flagellum and cilliim.
What type of cells (animal, plant, prokaryotic, etc.) are intermediate filaments typically found in?
Animal cells, specifically vertebrates.
Explain intermediate filaments structure and how they differe from the other 2 cytoskeletal structures.
- They are nonpolar, unlike the other 2 , meaning both of their ends are chemically identical (explaining their un-involvement in motility in the cell).
- More stable, resist more tension, less prone to assemble and dissemble quickly.
- Composed of 70+ proteins; whereas the other 2 have 1 exclusive protein that they are made of
- rope like, similar to microfilaments
Explain the function of intermediate filaments.
They are responsible for reinforcing the shape of the cell, as well as keeping various organelles in place.
-They make up the nuclear lamina, as well as they hold the nucleus in place from the outside.
What are glycoproteins and glycolipids and where are they typically found in the cell?
They are lipids or proteins with polysaccharides attached. They are common in the extracellular matrix. (made in Golgi)
What is a tight cell junction?
When the membranes of surrounding cells ae almost “fused”, preventing any extracellular fluids to seep through (impermeable).
Helpful in intestinal cells.
What are desmosomes?
Known as anchoring junctions between cells; they fasten cells together in tight sheets, held in place in the cell by intermediate filaments.
What are gap junctions?
Also called “signalling junctions”, they are cytoplasmic channels between cells. They can allow molecules or ions to pass between cells via channels, important for food and communicating purposes.
How do monomers generally bond together?
Through a condensation reaction (also known as dehydration synthesis), where a water molecule is produce when the bond is formed.
Where do most of the reaction involving breaking up polymers/building up polymers take place?
The lysosome.
What is the difference between alpha and beta glucose?
Alpha glucose and beta glucose have alternated hydroxyl groups on the first carbon. (in alpha glucose the hydroxyl is facing down, and in beta it is facing up).
What is the main function of polysaccharides?
Our body can either harvest energy from them through eating, or they can store energy through them.
What is glycogen and what is its function?
-Glycogen is a polysaccharide composed of glucose molecules, and is the main energy storage unit for animals.
-Animals store their carbohydrates as this and break it up via hydrolysis reactions when needed.
Its a long, branched molecule.
What is starch, and what is its function?
- Starch is a polysaccharide of glucose, and has 2 forms: amylose and amylopectin. Amylose is a non branched chain with 1-4 linkages, whereas amylopectin is slightly branched with 1-4 linkages and then 1-6 linkages for branches.
- This is the energy storage unit for plants, and is what provides animals of their glucose content.
What is cellulose and what is its function?
Cellulose is used for structure in plant cells (i.e. plant cell walls). It is composed of beta-glucose molecules, preventing most animals from digesting it.
This allows it to serve as a source of fibre.
What is the bond between two monosaccharides called?
A glycosidic linkage.
What are the 3 common disaccharide and what are their monomers?
(1) Maltose-2 glucose
(2) Surcose- glucose + fructose
(3) Lactose- galactose + glucose
What is the empirical formula for a carbohydrate?
1:2:1 (c1:h2:o1)
Explain the structure of a triglyceride, and its function.
A triglyceride is composed of 1 glycerol molecule, bonded to 3 fatty acid chains via hydrolysis.
The bond between the glycerol and fatty acids is known as an ester linkage.
This type of fat is what makes up the visible fat on your body, for warmth and protection.
What is a saturated vs. unsaturated fat (fatty acid) ?
- Saturated fats contain no double bonds between their carbons, whereas unsaturated fats have at least 1 or more.
- The double bond in a fatty acid causes the tail to kink, preventing the tails from locking together as tightly. This increases the fluidity of the fat (liquid at room temp), allowing for easier breakdown than the saturated fat.
Where are saturated fats found? Where are unsaturated fats found?
Saturated fats come from mainly animals, where as unsaturated fats come mainly from plants and oils.
What are the 2 types of unsat fats?
Cis an trans, referring to the branching pattern after the double bond. Cis unsat fats are the fluid ones, that cause a kink in the chain. Trans fats, on the other hand, are almost exactly the same as saturated fats.
How were trans fats created?
Unsat fats have a relatively short shelf life, so people discovered they can be hydrogenated to form saturated fats with longer shelf lives, but trans fats were a byproduct of this.
Trans fat have a very long shelf life, and taste very good, but are terrible for human health.
What is the structure and function of a phospholipid?
- Composed of a glycerol molecule bonded to 2 fatty acid chains, and one phosphate. This phosphate is then bonded to an r-group.
- Component of cell membranes due to its amphipathic property.