BI203 Lecture 17: Protein Sorting and Transport - Slides 37-64 (Golgi Apparatus and Vesicular Transport) Flashcards
Protein transit from ER to final destination is mediated by ___ transport.
vesicular
Vesicular transport entails ___ steps:
four
Vesicular transport entails four steps:
1) Sorting of ___ into a vesicle bud.
2) Vesicle budding from the ___ ___ ___ (ERES).
3) Vesicle ___ to the target membrane.
4) Vesicle ___ with the target membrane.
cargo, ER Exit Site (ERES), docking, fusion
Vesicles fuse to form the ___ ___ ___ (ERGIC), then move to the Golgi apparatus.
ER-Golgi Intermediate Compartment (ERGIC)
Proteins targeted for export have ___ and ___ signals that direct their packaging into transport vesicles.
peptide, carbohydrate
Unmarked proteins in the ER can also be packaged and transported to the Golgi by a ___ pathway.
default
Proteins that function within the ER are recognized in the ___ or Golgi and transported back to the ER.
ERGIC
ER transmembrane proteins, such as BiP, have a targeting sequence (KDEL or KKXX) at the carboxyl terminus that directs retrieval back to the ___.
ER
ER transmembrane proteins, such as BiP, have a targeting sequence (___ or ___) at the carboxyl terminus that directs retrieval back to the ER.
KDEL, KKXX
___ ___ - Proteins from the ER are processed and sorted for transport to endosomes, lysosomes, the plasma membrane, or secretion.
Golgi Apparatus
Most glycolipids and sphingomyelin are synthesized in the ___ ___.
golgi apparatus
The Golgi is composed of flattened membrane-enclosed sacs (___) and associated vesicles.
cisternae
The golgi apparatus also has ___.
polarity
Golgi Apparatus polarity:
Proteins from the ER enter at the ___ face, usually oriented toward the ___. They exit from the ___ face.
cis, nucleus, trans
Golgi Apparatus polarity:
Proteins from the ER ___ at the cis face, usually oriented toward the nucleus. They ___ from the trans face
enter, exit
The Golgi Apparatus has ___ compartments.
4
The Golgi Apparatus Compartments:
___ compartment - receives molecules from the ERGIC.
Cis
The Golgi Apparatus Compartments:
___ and ___ compartments - most modifications are done here.
Medial, Trans
The Golgi Apparatus Compartments:
The ___ ___ - the sorting and distribution center.
Trans-Golgi Network
___ different models for how proteins move through the Golgi Apparatus.
2
The ___ ___ model: Proteins are carried between cisternae in transport vesicles.
stable cisternae model
The ___ ___ model: Proteins are carried within the cisternae, which gradually mature and progressively move through the Golgi in the cis to trans direction.
Vesicles then return Golgi resident proteins back to earlier Golgi compartments.
cisternal maturation model
The stable cisternae model: Proteins are carried between ___ in transport vesicles.
cisternae
The cisternal maturation model: Proteins are carried within the cisternae, which gradually mature and progressively move through the Golgi in the ___ to ___ direction.
Vesicles then return Golgi resident proteins back to earlier Golgi compartments.
cis, trans
