BI203 Lecture 17: Protein Sorting and Transport - Slides 37-64 (Golgi Apparatus and Vesicular Transport)

Question 1

Protein transit from ER to final destination is mediated by ___ transport.

Answer 1

vesicular

Question 2

Vesicular transport entails ___ steps:

Answer 2

four

Question 3

Vesicular transport entails four steps:

1) Sorting of ___ into a vesicle bud.
2) Vesicle budding from the ___ ___ ___ (ERES).
3) Vesicle ___ to the target membrane.
4) Vesicle ___ with the target membrane.

Answer 3

cargo, ER Exit Site (ERES), docking, fusion

Question 4

Vesicles fuse to form the ___ ___ ___ (ERGIC), then move to the Golgi apparatus.

Answer 4

ER-Golgi Intermediate Compartment (ERGIC)

Question 5

Proteins targeted for export have ___ and ___ signals that direct their packaging into transport vesicles.

Answer 5

peptide, carbohydrate

Question 6

Unmarked proteins in the ER can also be packaged and transported to the Golgi by a ___ pathway.

Answer 6

default

Question 7

Proteins that function within the ER are recognized in the ___ or Golgi and transported back to the ER.

Answer 7

ERGIC

Question 8

ER transmembrane proteins, such as BiP, have a targeting sequence (KDEL or KKXX) at the carboxyl terminus that directs retrieval back to the ___.

Answer 8

ER

Question 9

ER transmembrane proteins, such as BiP, have a targeting sequence (___ or ___) at the carboxyl terminus that directs retrieval back to the ER.

Answer 9

KDEL, KKXX

Question 10

___ ___ - Proteins from the ER are processed and sorted for transport to endosomes, lysosomes, the plasma membrane, or secretion.

Answer 10

Golgi Apparatus

Question 11

Most glycolipids and sphingomyelin are synthesized in the ___ ___.

Answer 11

golgi apparatus

Question 12

The Golgi is composed of flattened membrane-enclosed sacs (___) and associated vesicles.

Answer 12

cisternae

Question 13

The golgi apparatus also has ___.

Answer 13

polarity

Question 14

Golgi Apparatus polarity:

Proteins from the ER enter at the ___ face, usually oriented toward the ___. They exit from the ___ face.

Answer 14

cis, nucleus, trans

Question 15

Golgi Apparatus polarity:

Proteins from the ER ___ at the cis face, usually oriented toward the nucleus. They ___ from the trans face

Answer 15

enter, exit

Question 16

The Golgi Apparatus has ___ compartments.

Answer 16

4

Question 17

The Golgi Apparatus Compartments:

___ compartment - receives molecules from the ERGIC.

Answer 17

Cis

Question 18

The Golgi Apparatus Compartments:

___ and ___ compartments - most modifications are done here.

Answer 18

Medial, Trans

Question 19

The Golgi Apparatus Compartments:

The ___ ___ - the sorting and distribution center.

Answer 19

Trans-Golgi Network

Question 20

___ different models for how proteins move through the Golgi Apparatus.

Answer 20

2

Question 21

The ___ ___ model: Proteins are carried between cisternae in transport vesicles.

Answer 21

stable cisternae model

Question 22

The ___ ___ model: Proteins are carried within the cisternae, which gradually mature and progressively move through the Golgi in the cis to trans direction.
Vesicles then return Golgi resident proteins back to earlier Golgi compartments.

Answer 22

cisternal maturation model

Question 23

The stable cisternae model: Proteins are carried between ___ in transport vesicles.

Answer 23

cisternae

Question 24

The cisternal maturation model: Proteins are carried within the cisternae, which gradually mature and progressively move through the Golgi in the ___ to ___ direction.
Vesicles then return Golgi resident proteins back to earlier Golgi compartments.

Answer 24

cis, trans

Question 25

Protein transport from the ER to Golgi Apparatus:

When a vesicle buds from the ER, the ___ of the membrane leaflets is maintained. (___ domain vs. ___ domain).

Answer 25

orientation, cytosolic, lumenal

Question 26

Protein transport from the ER to Golgi Apparatus:

The vesicles fuse to form the ___ ___ ___ (ERGIC), then move to the Golgi apparatus.

Answer 26

ER-Golgi Intermediate Compartment (ERGIC)

Question 27

Protein transport from the ER to Golgi Apparatus:

Once again, when the vesicle fuses at the Golgi Apparatus, the ___ of the membrane leaflets is maintained.

Answer 27

orientation

Question 28

Protein transport from the Golgi Apparatus to Cell Exterior:

And once again, when the secretory vesicle fuses with the plasma membrane, the ___ of the membrane leaflets is maintained:

The cytosolic domain stays the cytosolic domain.
The lumenal domain becomes the ___ domain.

Answer 28

orientation, extracellular