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UA & BF > BF MAMC > Flashcards

BF MAMC Flashcards

1
Q

Whats the dilution factor used for sperm motility

A

1: 20

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2
Q

what’s the solution used to dilute sperm for motility

A

PBS - Phosphate buffer solution

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3
Q

What does semen analysis includ

A 
volume
color
viscosity
liquefaction
motility
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4
Q

what’s the reference range for motility for semen

A

> 49%

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5
Q

when is motility of a semen sample read

A

within 1 hour of collection

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6
Q

Red cell diluent

A

saline

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7
Q

Diluent that destroys red cells and enhances wbcs

A

3% glacial acetic acid

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8
Q

for CSF analysis, what tubes are used to rule out bleeding

A

1 and 4

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9
Q

When do we count tube 1 and 4

A

when the csf is xanthochromic or grossly bloody

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10
Q

What tube do we count first for CSF

A

4

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11
Q

what tube do we used to perform cell count

A

4 if clear

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12
Q

When do we perform cell count on tube 4

A

when RBCs is >/10 or WBC >/4

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13
Q

when do prepare cytospin (differential) for cell count

A

if WBCs is >4

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14
Q

where is CSF produced

A

in choroid plexus

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15
Q

how are the malignant cells in CSF called

A

choroid plexus

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16
Q

what’s the function of CSF

A

provides nutrients to css
moves metabolic waste
mechanical barrier to cushion

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17
Q

Whats require for CSF samples

A

total volume

clarity/clot

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18
Q

what do we do we bloody CSF

A

spin down, if it does not clear is xathochromic

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19
Q

When do we perform WBC count on normal CSF

A

when heme or Onco patient

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20
Q

when do we perform cell count in tube 2 for CSF

A

leukemia/cancer

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21
Q

when do we perform cell count in tube 3 for CSF

A

to rule out meningitis

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22
Q

What does xanthochromsia indicates

A

traumatic tap, intracerebral or subarachnoid hemorrhage

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23
Q

How do we perform a cell count in CSF

A

1 drop of albumin + 2 or 3 drops of CSF

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24
Q

What cells can we see in CSF

A

Neutrophil, Lymph, Eso, baso, Mono macro

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25
Q

a macro/mono with iron in the cytoplasm

A

hemosiderin

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26
Q

characteristic of choroid plexus

A 
clumping
larger than normal 
dark color
phagocytosis
bledding
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27
Q

What do we used to collect synovial fluid

A

a purple tap or a syringe with EDTA

28
Q

How do you lyse RBC in synovial fluid

A

hyaluronidase

29
Q

How do you eliminate viscosity of synovial

A

hyaluronidase, saline dilution or saline wash

30
Q

how do we check for crystal in synovial fluid

A

using a wet prep

31
Q

what are the two type of crystal in synovial fluid

A

MSU and CPPD

32
Q

what does MSU mean

A

Monosodium urate crystals

33
Q

what does cppd mean

A

Calcium Pyrophosphate Deposition

34
Q

what type of crystal is msu

A

gout

35
Q

what type of crystal is cppd

A

pseudogout

36
Q

what analyses we used to do cell count on synovial fluid

A

sysmex

37
Q

what do we do we normal results for synovial using sysmex

A

we release

38
Q

what do we do with

A

perform manual count

39
Q

how do we perform count in body fluids

A

using a cytospin

40
Q

what’s a cytospin

A

concentrate cells in fluid

41
Q

how do we prepare a slide for synovial

A

cytospin + one drop

42
Q

what cells do we see in synovial

A

Neutrophil, Lymph, Eso, baso
Mono
macro
synovial cell

43
Q

where do we see choroid plexus

A

in csf

44
Q

when do we see plasmacyte

A

all, specially black

45
Q

when do we see hemosideron

A

all fluids except synovial

46
Q

where do we see cholesterol crystal

A

in synovial fluid

47
Q

when do we see epithelial cell

A

in bla

48
Q

what are the different type of serous fluid

A 
pericardial
pleural
ascites
thoracentesis
peritoneal
49
Q

when do we see mesothelia cell

A

in serous fluid

50
Q

a macrophage with a nucleus push to the side (like a half moon)

A

signet ring

51
Q

a macrophage with RBCs inside

A

erythro phagocytosis

52
Q

what abnormal macrophages can we see in serous fluid

A

hemosiderin
meso
signet ring
erythro phagocytosis

53
Q

what cells are included in a serous cell count

A

neutro, lymp, eso, baso and macro

54
Q

are there monos in serous fluid

A

yes but they are called macro

55
Q

BLA

A

washing out lungs with saline

56
Q

is there mono in bla

A

nooooooooo

57
Q

characteristics of bronchial epic

A

light city
cilia
nucleus

58
Q

what happen when you centrifuge pseudochylous sample

A

they become clear

59
Q

what happen when you centrifuge chylous fluid

A

still milky

60
Q

Stain used for: Differential

A

Wright stain

61
Q

Stain used for: PCP

A

Silver Nitrate Stain

62
Q

Stain used for: Lipid Laden

A

Oil Red O

63
Q

Stain used for: Nasal smear and urine for eos

A

Hansel stain

64
Q

Stain used for: Urine for hemosiderin

A

Iron stain or Perls stain

65
Q

Stain used for: semen analysis

A

5% eosin Y and 10% nigrosin

66
Q

Sperm morphology

A 
ovals
doubles
tail defects
immature
amorphous

UA & BF (3 decks)

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