BF MAMC Flashcards
Whats the dilution factor used for sperm motility
1: 20
what’s the solution used to dilute sperm for motility
PBS - Phosphate buffer solution
What does semen analysis includ
volume color viscosity liquefaction motility
what’s the reference range for motility for semen
> 49%
when is motility of a semen sample read
within 1 hour of collection
Red cell diluent
saline
Diluent that destroys red cells and enhances wbcs
3% glacial acetic acid
for CSF analysis, what tubes are used to rule out bleeding
1 and 4
When do we count tube 1 and 4
when the csf is xanthochromic or grossly bloody
What tube do we count first for CSF
4
what tube do we used to perform cell count
4 if clear
When do we perform cell count on tube 4
when RBCs is >/10 or WBC >/4
when do prepare cytospin (differential) for cell count
if WBCs is >4
where is CSF produced
in choroid plexus
how are the malignant cells in CSF called
choroid plexus
what’s the function of CSF
provides nutrients to css
moves metabolic waste
mechanical barrier to cushion
Whats require for CSF samples
total volume
clarity/clot
what do we do we bloody CSF
spin down, if it does not clear is xathochromic
When do we perform WBC count on normal CSF
when heme or Onco patient
when do we perform cell count in tube 2 for CSF
leukemia/cancer
when do we perform cell count in tube 3 for CSF
to rule out meningitis
What does xanthochromsia indicates
traumatic tap, intracerebral or subarachnoid hemorrhage
How do we perform a cell count in CSF
1 drop of albumin + 2 or 3 drops of CSF
What cells can we see in CSF
Neutrophil, Lymph, Eso, baso, Mono macro
a macro/mono with iron in the cytoplasm
hemosiderin
characteristic of choroid plexus
clumping larger than normal dark color phagocytosis bledding
What do we used to collect synovial fluid
a purple tap or a syringe with EDTA
How do you lyse RBC in synovial fluid
hyaluronidase
How do you eliminate viscosity of synovial
hyaluronidase, saline dilution or saline wash
how do we check for crystal in synovial fluid
using a wet prep
what are the two type of crystal in synovial fluid
MSU and CPPD
what does MSU mean
Monosodium urate crystals
what does cppd mean
Calcium Pyrophosphate Deposition
what type of crystal is msu
gout
what type of crystal is cppd
pseudogout
what analyses we used to do cell count on synovial fluid
sysmex
what do we do we normal results for synovial using sysmex
we release
what do we do with
perform manual count
how do we perform count in body fluids
using a cytospin
what’s a cytospin
concentrate cells in fluid
how do we prepare a slide for synovial
cytospin + one drop
what cells do we see in synovial
Neutrophil, Lymph, Eso, baso
Mono
macro
synovial cell
where do we see choroid plexus
in csf
when do we see plasmacyte
all, specially black
when do we see hemosideron
all fluids except synovial
where do we see cholesterol crystal
in synovial fluid
when do we see epithelial cell
in bla
what are the different type of serous fluid
pericardial pleural ascites thoracentesis peritoneal
when do we see mesothelia cell
in serous fluid
a macrophage with a nucleus push to the side (like a half moon)
signet ring
a macrophage with RBCs inside
erythro phagocytosis
what abnormal macrophages can we see in serous fluid
hemosiderin
meso
signet ring
erythro phagocytosis
what cells are included in a serous cell count
neutro, lymp, eso, baso and macro
are there monos in serous fluid
yes but they are called macro
BLA
washing out lungs with saline
is there mono in bla
nooooooooo
characteristics of bronchial epic
light city
cilia
nucleus
what happen when you centrifuge pseudochylous sample
they become clear
what happen when you centrifuge chylous fluid
still milky
Stain used for: Differential
Wright stain
Stain used for: PCP
Silver Nitrate Stain
Stain used for: Lipid Laden
Oil Red O
Stain used for: Nasal smear and urine for eos
Hansel stain
Stain used for: Urine for hemosiderin
Iron stain or Perls stain
Stain used for: semen analysis
5% eosin Y and 10% nigrosin
Sperm morphology
ovals doubles tail defects immature amorphous
