Benedicts test for non reducing sugars

Question 1

What disaccharide can you use non reducing sugars test on?

Answer 1

Sucrose

Question 2

Steps for non reducing sugars test

Answer 2

1. Take a small sample and heat in 95’C water baths with benedicts solution to confirm it’s negative result
2. Hydrolyse another sample of the sucrose by heating in 95’C water baths with dilute acid
3. When cooled, neutralise acid with alkali
4. Add same concentration of Benedicts solution and heat in 95’C water bath.
5. Positive brick red colour indicates a non-reducing sugar was originally present

Question 3

Positive result for presence of non reducing sugar

Answer 3

Brick red colour