Belton Quiz (Dilutions)

Question 1

State the dilution factor formula

Answer 1

D.F. = V2/V1 = C1/C2

Once you find V2 needed to dilute, do V2 - V1 and add that much of water, and let the difference be the stock you are diluting

Question 2

Describe serial dilutions

Answer 2

100 D.F. = 10 uL

10 D.F. = 100 uL

Question 3

How do you find cells/mL given volume of plate and number of colonies?

Answer 3

1 colony = 1 cell

Cells/mL x dilution concentration