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HTMLE > BE HTMLE > Flashcards

BE HTMLE Flashcards

1
Q

Best fixative for the nervous system

A

FORMALIN FIXATIVE

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2
Q

Fixative of choice for the preservation of fats

A

Formalin fixative

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3
Q

Helly s fluid is exactly the same as in Zenker’ s fixative, but instead of GLACIAL ACETIC ACID, what component is added

A

Formalin

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4
Q

Fixative recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei

A

Zenker Fluid

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5
Q

Excellent microanatomic fixative for pituitary gland, bone marrow and blood containing organs such as spleen and liver

A

Hellys

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6
Q

All mercurial fixing solutions lead to the formation in tissues of diffuse BLACK granules and these mercury deposits must be removed BEFORE STAINING. Removal of mercuric chloride deposit is accomplished by

A

Saturated solution of iodine

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7
Q

Considered to be the MOST RAPID fixative/ recommended for fixing chromosomes, lymph glands and urgent biopsies:

A

Carnoys

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8
Q

Most widely used fixative for electron microscopy

A

Lead fixative

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9
Q

The fastest chemical solution in decalcifying tissues is:

A

Nitric acid

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10
Q

All of the following remove intracellular and extracellular water from the tissue following fixation, EXCEPT:
A. Alcohol
C. Tetrahydrofuran
B. Chloroform
D. Dioxane

A

B

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11
Q

A TOXIC dehydrating agent, primarily employed for blood and tissue films and for smear preparation:
A. Ethyl alcohol
C. Butyl alcohol
B. Methyl alcohol
D. Isopropyl alcohol

A

B

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12
Q

Function/s of tetrahydrofuran:

A

Dehyrating agent Clearing agent

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13
Q

All of the following are substitutes for paraffin wax, EXCEPT:
A. Paraplast
B. Embeddol
C. Malinol
D. Tissue Mat

A

C

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14
Q

Melting point of ester wax:

A

46-48C

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15
Q

Microtome knife recommended for frozen sections or for cutting extremely hard and tough specimens embedded in paraffin blocks, using a base-sledge type or sliding microtome:

A

Plane wedge knife

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16
Q

Angle formed between the cutting edge of the microtome knife
(27o-32o)

A

Bevel angle

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17
Q

Angle formed between the surface of the block and the cutting edge of the knife (0o-15o)

A

Clearance angle

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18
Q

This type of microtome easily cuts large blocks and serial sections can be obtained with ease because larger knives can be used

A

Sliding

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19
Q

A tissue exposed to short burst of CARBON DIOXIDE for a few minutes will

A

Freeze

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20
Q

Fat cells and enzymes are best demonstrated in:

A

Frozen section

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21
Q

When trimming tissue block, they must be surrounded by at least __ of wax

A

2mm

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22
Q

The following methods are done for drying sections on slide, EXCEPT:
A. On a hot plate at 45-55oC for 30-45 minutes
B. On a Bunsen flame
C. In an incubator at 37oC for 3 hours
D. In a wax oven at 56-60oC for 2 hour

A

C

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23
Q

Adhesive added to the water in the floating-out bath most convenient alternative to direct coating of slide

A

Gelatin

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24
Q

Process by which sections are stained with simple aqueous or alcoholic solutions of the dye:

A

Direct staining

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25
Q

A tissue-mordant-dye complex is needed in

A

Indirect staining

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26
Q

With this staining technique, the tissue is first overstained, and the excess stain is removed or decolorized from unwanted part of the tissue

A

Regressive staining

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27
Q

All of the following are metachromatic stains, EXCEPT:
A. Thionine
C. Safranin
B. Toluidine blue
D. Eosin

28
Q

Stain used for demonstrating mitochondria during intravital staining

A

Janus Green B

29
Q

Application of a different color or stain to provide contrast and background to the staining of the structural component to be demonstrated

A

Counterstaining

30
Q

The routine stain for surgical tissue section is:

31
Q

Routinely used in histopathology as a counterstain/background fro contrasting stain because it gives a pleasing and colorful contrasts to nuclear stains

32
Q

In routine H&E, most fixatives can be used, EXCEPT:
A. Zenker s formol
C. Osmic acid
B. Formaldehyde
D. Picric acid

33
Q

Only substance in histopathology that can fix, differentiate or stain tissues all by itself:

A

Picric acid

34
Q

The active dye (coloring agent) in hematoxylin solution is

35
Q

All of the following are chemical oxidizing agent/ripening agent for hematoxylin, EXCEPT:
A. Sodium iodate
C. Hydrogen peroxide
B. Mercuric oxide
D. Ammonium alum

36
Q

Ripening agent for Harris hematoxylin

A

Mercuric oxide

37
Q

Function of ammonium alum in the Harris hematoxylin formula:

38
Q

Staining of the nuclei by alum hematoxylin is enhanced by the addition of:

A

Glacial acetic acid

39
Q

A sheen of oxidized dye on the surface of hematoxylin solution indicate

A

stain has to be filtered

40
Q

The acid used in combination with alcohol in an acid alcohol solution

A

Hydrochloric acid

41
Q

Staining result in the routine hematoxylin and eosin method

A

Nuclei blue to blue-black, cytoplasm pink

42
Q

A common BASIC NUCLEAR STAIN for plasma cells and may also be employed in cytological examination of fresh sputum for malignant cells:

A

Methylene blue

43
Q

PAS positive substances are stained

A

Magenta red

44
Q

Staining method for glycogen:
A. PAS technique
C. Best Carmine method
B. Langhan s iodine stain
D. All of these

45
Q

Tubercle bacilli in Ziehl-Neelsen stain:

A

Bright red

46
Q

Feulgen s reaction is the most reliable and most specific histochemical staining technique for:

47
Q

In the Masson-Fontana ammoniacal silver reaction, melanin and argentaffin cell granules are stained

48
Q

Positive reaction for cholesterol in Schultz Method:

A

Blue-green

49
Q

Components of the EA-50 stain, EXCEPT:
A. Eosin Y
C. Light green
B. Orange green
D. Bismarck brown

50
Q

All of the following are fixative used for cytologic smears, EXCEPT:
A. Equal parts of 95% ETOH and ether
C. 10% Formalin
B. 95% Ethyl alcohol
D. Carnoy s fluid

51
Q

BEST fixative for virtually all diagnostic cytology studies but flammable and volatile

A

Equal parts of 95% ETOH and ether

52
Q

All of the following are examples of good adhesive agents for cytologic method, EXCEPT:
A. Egg albumin
C. Pooled human serum or plasma
B. Celloidin ether alcohol
D. Leuconostoc culture

53
Q

Smears prepared for cytologic examination from cut surfaces of lymph nodes can be obtained by

A

Touch preparation

54
Q

Organisms found in the normal vaginal flora that stain BLUE to lavander with Pap s method:

A

Doderlain bacilli

55
Q

Cells found in a vaginal smear which are thick and round to oval in shape (similar to fried fresh eggs with sunny-side up) with strongly basophilic cytoplasm and vesicular nucleus

56
Q

Increase in size of an organ or tissue due to an increase in number of cells

A

Hyperplasia

57
Q

The dissolving of cells by enzymatic action is

58
Q

A malignant tumor is least characterized by:
A. Metastases
C. Encapsulation
B. Mitotic figures
D. Invasion of adjacent tissues

59
Q

The Blood Banking Law

60
Q

National Blood Services Act of 1994:

61
Q

Philippine AIDS Prevention and Control Act of 1998

62
Q

Newborn Screening Act of 2004

63
Q

All services are done on a primary category laboratory except: (A.O. 2007-0027)
A. Routine urinalysis
C. Routine chemistry
B. Routine hematology D. Blood typing

64
Q

Who are the members of the Medical technology Board?

A

A registered pathologist and two registered medical technologist

65
Q

Sections of RA 5527 amended by PD 1534

66
Q

The penalty of revocation of certificate of registration may be imposed by the board if there is

A

Unanimous vote

HTMLE (6 decks)

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