BCHM

Question 1

A positive hydropathy index means the a.a are

Answer 1

hydrophobic - mor likely to bein PL - transmembrane NP - related to the number of spikes/stretches

Question 2

what is Km

Answer 2

Km is the concentration of substrate which permits the enzyme to achieve half Vmax.

Question 3

What is the DV in experiment that determines the MM kinetic parameters

Answer 3

initial velocity

Question 4

how many molecules of NADH produced from 6 molecules of glucose that undergo glycolysis

Answer 4

12 - 2 NADH each glucose

Question 5

lipid with unsat chains at high temp causes (disorder or order)

Answer 5

disorder

Question 6

what happens when a G protein coupled receptor is activated

Answer 6

the alpha subunit binds to GTP

Question 7

What is least likely to happen during apop and likely

Answer 7

NOT: damage to nearby cells causes by lysis( necrosis) but they DO: cause cytochrome c release, caspase activation, cell shrinkage, and blebbing

Question 8

which compound is NOT a component of CAC

Answer 8

pyruvate

Question 9

a.a. with the highest isoelectric point

Answer 9

arginine - most basic, has positive charge

Question 10

which a.a form H bond with glutamate (-)

Answer 10

has to be one with hydrogen donor group - gln - not asp because opposing neg charges

Question 11

uncompetivie inhibito parameters

Answer 11

low, low

Question 12

slope of the lineweaver plot is

Answer 12

Km/Vmax

Question 13

formula of MM equation

Answer 13

Vmax S/ Km + S

Question 14

catalytic efficiency of an enzyme is given

Answer 14

by kcat/kM

Question 15

specific activity is a measure of

Answer 15

the enzyme units per milligram of total protein in solution

Question 16

with size exclusion chroma u have 10 kda, 100 kda, and 40 kda, and urea which will elute last

Answer 16

urea - smallest - NH3 - just molecues not a,a

Question 17

compared to a diunsat acyl chain of a lipid a saturated acyl chain has how many more H atoms

Answer 17

4

Question 18

ex. as Phe is hydrophobic group found in/out if protein and to go onto the exterior it will require more energy (pos G)

Answer 18

in the interior

Question 19

disruption of 2 disulfide bonds within a protein requires how many NADH (reducing agent)

Answer 19

2 molecules

Question 20

to be a better nucleophile you have ______ and with methionines exampe is

Answer 20

need elctrons )negative) in Met ut has Sulfur which has electrons