BCH 184 Spectroscopy Flashcards
What are the criteria for method choice in Spectroscopy?
Sensitive : Needs small amount of material
Selective: can report on the function of interest
Resolution: can be potentially high
Non-destructive: or minimally destructive to the thing youre measuring
Light wave energy equation?
E=hc/λ
Boltzman Distribution?
N_2/N_1 =e^((−∆E)⁄(k_B T))
Einstien relation for emissions?
A_21/B_21 =(8πhv^3)/c^3
What determines if spontaneous or stimulated emissions dominate?
If hv»_space;kt spontaneous emissions dominate
hv «kt stimulated emissions dominate
What determines the probability of transition?
The extent of the charge redistribution and the overlap between the ground and excited state wave functions
What do these terms mean?
What is the equation that shows Short excited state lifetimes result in large linewidths?
what is the equation that shows how pressure broadening effects lorentzian broadening?
What is the equation to determine if a molecule is optically active?
What is the equation for determining Nucleic acid duplex stability?
What two amino acids must be present in a protein in order for it to be visible in absorption tests?
Tryptophan and Tyrosine
What does the transition dipole moment define?
The extent of the charge redistribution during the transistion
How does Pi-bond conjugation typically affect the maximum absorbance wavelength and Molar absorptivity?
Increasing Pi-bond conjugation typically increase both maximum absorbance wavelength and Molar Absorptivity
What form does homogeneous broadening take?
Lorentzian line shapes with a FWHM of 1/piT_rad
