BC Practice Ex. 2 Flashcards
What metal ion is critical in muscle contraction?
Ca2+
Which statement about myoglobin is FALSE?
It has 4 sites to bind to oxygen
What is the first step in immunoblotting?
Protein separation by gel electrophoresis
The heme prosthetic group….
consists of protoporphyrin and an iron (II) ion.
Which statement is FALSE regarding the binding of 2,3-BPG to hemoglobin?
BPG blood levels decrease in individuals shortly after they move from sea level to
higher altitudes.
An arrow points to part of the immunoglobulin structure. What is the name of this
portion of the structure?
Fab
Which experiment demonstrated that enzymes are proteins?
isolation and crystallization of urease
A regulatory enzyme whose activity is affected/controlled by the reversible, noncovalent
binding of small metabolites or other compounds at a site other than the enzyme’s active
site is called an _____ enzyme
Allosteric
Consider the reaction:
E + S ⇌ ES ⇌ EP ⇌ E + P
What do ES and EP represent in the reaction?
reaction intermediates consisting of transient complexes of the enzyme with the
substrate and with the product, respectively
What is the steady state?
the reaction period where [ES] is constant
What is a major advantage of a Lineweaver-Burk plot over a Michaelis-Menten plot?
It is easier to determine Vmax from a y intercept than from an asymptote.
Compared to an uncatalyzed reaction, what does an enzyme change?
Energy of activation
Which class of enzymes relocates molecules or ions across membranes?
Translocase
What is the current theory behind the substrate enzyme interaction in the enzymes active site?
The active site is complementary to the substrate. Xxxx or transition
What is glycobiology?
Study of glycoconjugates
