Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

CCP3.1 > Basics of Chromatography > Flashcards

Basics of Chromatography Flashcards

1
Q

the ability to separate molecules using various partitioning characteristics of molecule to remain in a stationary phase versus a mobile phase.

A

Chromatoraphy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Once a molecules is separate from teh mixture it can be _______ and _______.

A

isolated and quantified

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Can chromatography identify components?

A

No, it needs a detector

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

In liquid chromatography detector response is ____ a determeing factor for the colum and thin layer efficiencies

A

Not

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Who applied chromatography first in the separation of plant pigments?

A

Mikhail Semyonovich Tsvet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Greek for color

A

chromatos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Greek for to write

A

graphein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the major components of chromatography separation

A

Mobile Phase
Stationary Phase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

flows through the column; carries analyte

A

Mobile Phase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

stays in place, does not move

A

Stationary Phase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

instrument employed for chromatography

A

Chromatograph

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Phase that stains in place inside the collumn.

A

Stationary phase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

It can be a
particular solid or gel-based packaging
(LC) or a highly viscous liquid coated
inside the column (GC)

A

Stationary Phase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

the solvent moving
through the column; either a liquid in LC
or gas inGC.

A

Mobile Phase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

fluid entering the column.

A

Eluent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

fluid exiting the column.

A

Eluate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

is the process of passing the mobile
phase through the column.

A

Elution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

graph showing detector
response as a function of time.

A

Chromatogram

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

defines how much mobile phase
passed per minute (mL/min)

A

Flow Rate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

distance passed by mobile
phase per 1 min in the column (cm/min)

A

Linear Velocity

21
Q

What are the types of Chromatography?

A

Based on the Bed
Based on the Physical State Phases
Based on the Separation Mechanism

22
Q

Enumerate the based on the Bed chromatography

A

Column Chromatography
Planar Chromatography

23
Q

Enumerate the chromatography based on the Physical State phases

A

Gas chromatography
Liquid Chromatography

24
Q

Enumerate the chromatography based on the Separation mechanism

A

Affinity chromatography
Ion-exchange chromatography
Gel-Filtration chromatography

25
Q

Enumerate the chromatography in Planar chromatography

A

Paper chromatography
Thin-layer chromatography

26
Q

Enumerate the chromatography in liquid chromatography

A

High-performance liquid chromatography
Super critical fluid chromatography

27
Q

Enumerate the chromatography in Ion-exchange chromatography

A

Anion-exchange chromatography
Cation-exchange chromatography

28
Q

What are the types of Chromatography on the basis of interaction of Analyte with Stationary phase?

A

Adsorption
Ion exchange
Partition
Size Exclusion
Affinity

29
Q

of solute on surface of Stationary phase; for polar non-ionic compounds

A

adsorption

30
Q

attraction of ions of opposite charges; for ionic compounds anions or cations

A

Ion exchange

31
Q

based on the relative solubility Analyte in mobile and Stationary phases

A

partition

32
Q

another name for size exclusion

A

gel filtration, gel permeation

33
Q

separates molecules by size; sieving - not real interaction, small molecules travel longer

A

Size exclusion

34
Q

Specific interactions like antibody to protein

A

Affinity

35
Q

Particles stick to the surface of the other phase

A

adsorption

36
Q

particles soak into the bulk of the other phase

A

absorption

37
Q

separates leaf pigments according to their size

A

leaf chromatography

38
Q

anthocyanins

A

Red

39
Q

carotenoids

A

orange

40
Q

xanthophyll

A

yellow

41
Q

chlorophyll

A

green

42
Q

small molecules travel ______ than large ones

A

further

43
Q

Formula for retention factor

A

Rf = dsolute/dsolvent front

44
Q

Enumerate the Rf values of:
Beta-carotene
Chlorophyll A
Chlorophyll B
Violaxanthin
Lutein

A

0.99
0.30
0.13
0.40
0.68

45
Q

The higher the Rf, the ___ polar is the solute

A

less

46
Q

the _____ the Rf, the more polar is the solute

A
47
Q

The higher the Rf, the ____ solublle is the solute

A

more

48
Q

The ____ lower the Rf, the less soluble is the solute

A

Lower

CCP3.1 (3 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions