BASICS Flashcards
First blood transfusion
1492- POPE INNOCENT VII
Recommend sodium phosphate as blood preservative
Braxton Hicks
Discover the ABO blood group system
Karl Landsteiner
Give the ISBT system number of ABO and Rh
First 3: Blood Group System
Last 3: Specific antigen
ABO: ISBT 004 001
Rh: ISBT 001 009
Most important of all blood group
Most common cause of HTR and HDN
Associated with IMMEDIATE, INTRAVASCULAR HEMOLYSIS
ABO
Specimen: Patient RBC
Known: antisera; anti-A (blue); anti-B (yellow)
Use: Detection of antigens
FORWARD TYPING/ front/ direct typing
Universal recipient
AB- no antibodies
Other names for reverse typing
Back/Indirect/Serum Typing
Specimen: Serum/Plasma
Reagent: A cells, B cells
Use: Detection of antibodies
REVERSE TYPING
Universal DONOR of whole blood or packed RBC
O- no antigen
Universal DONOR of PLASMA products
AB (no antibodies)
Grading Agglutination reactions
A few solid aggregates; mostly free floating cells; supernatant appears red
Mixed field
What is the immunodominant sugar of B
D-galactose
What is the glycosyltransferase of A
N-acetylgalactosyltransferase
What is the immunodominant sugar of A
N-acetyl-D-galactosamine