BASIC SEROLOGIC TURGEON 5TH ED

Question 1

1. A written procedural protocol should contain the following information, in the correct order: ____, ____, ____, ____. Choose from (A) to (D).
   A. Specimen collection and storage
   B. Reference values
   C. Reagents, supplies, and equipment
   D. Procedural method
   a. A, B, C, D
   b. B, C, A, D
   c. A, C, D, B
   d. D, C, B, A

Answer 1

c. A, C, D, B

Question 2

2. Factors that can denature, coagulate, or alter protein
   molecules include:
   a. Heat
   b. Strong acid solution
   c. Strong alkali solution
   d. All of the above

Answer 2

d. All of the above

Question 3

3. If testing cannot be done within ___hours of collection, a serum specimen should be frozen at
   −20° C.
   a. 24
   b. 48
   c. 72
   d. 96

Answer 3

c. 72

Question 4

4. Complement can be inactivated in human serum by heating to _____° C.
   a. 25
   b. 37
   c. 45
   d. 56

Answer 4

d. 56

Question 5

5. A specimen should be reinactivated when more than ______ hour(s) has (have) elapsed since inactivation.
   a. 1
   b. 2
   c. 4
   d. 8

Answer 5

c. 4

Question 6

6. A graduated pipette can be used when:
   a. Extreme accuracy is not needed.
   b. Very precise accuracy is needed.
   c. Precision is more important than speed.
   d. Precision and speed are important

Answer 6

a. Extreme accuracy is not needed

Question 7

7. A meniscus is the:
   a. Curvature in the top surface of a liquid
   b. Zero mark on a pipette
   c. Last marking on a serologic pipette
   d. Flat line of liquid in a pipette

Answer 7

a. Curvature in the top surface of a liquid

Question 8

8. Automatic pipettes have the advantage of:
   a. Being fast
   b. Allowing repetitive measurement of solutions
   c. Delivering equal volumes of solutions
   d. All the above

Answer 8

d. All the above

Question 9

9. A dilution is a(n):
   a. Ratio of volume or number of parts of the substance
   to be diluted in the total volume, or parts, of the final
   solution
   b. Indication of relative concentration
   c. Frequently used measure in serologic testing
   d. All the above

Answer 9

d. All the above

Question 10

10. If a serial dilution is prepared in 1:2 dilutions, the final
    dilution in tube 6 is:
    a. 1:25
    b. 1:32
    c. 1:64
    d. 1:256

Answer 10

c. 1:64

Question 11

11. and 12. To prepare 10 mL of a diluted serum specimen 1:10,
12. ___ part of serum is needed.
    a. 1.0
    b. 0.75
    c. 0.50
    d. 0.20
    and
13. ___ parts of distilled water is (are) needed to reach the
    total volume.
    a. 10
    b. 9
    c. 4.5
    d. 0.1

Answer 11

11. a. 1.0
    12.b. 9

Question 12

13. Serum for detection of antibodies should be drawnduring the:
    a. Acute phase of illness only
    b. Acute and convalescent phases of illness
    c. Convalescent phase of illness only
    d. Acute and convalescent phases, as well as 6 months after an illness

Answer 12

b. Acute and convalescent phases of illness

Question 13

14. A central concept of serologic testing is:
    a. Antigen-antibody interaction
    b. Determination of antibody composition
    c. Quantitation of antigen titer
    d. Manifestation of a rise in antibody titer

Answer 13

d. Manifestation of a rise in antibody titer