Basic Principles

Question 1

Enzyme responsible for conferring H activity on

the red cell membrane

Answer 1

a-L-fucosyltransferase

Question 2

Rejuvenation of a unit of RBCs is a method used to:

Answer 2

Restore 2,3-DPG and ATP to normal levels

Question 3

Process of separation of antibody from its antigen is known as:

Answer 3

Elution

Question 4

Movement of substances from an area of high to low concentration

Answer 4

Diffusion

Question 5

Removing antibodies from serum by incubation

Answer 5

Adsorption

Question 6

IgG antibodies react best at ___C.

Answer 6

37

Question 7

Which class of immunoglobulins is implicated in hemolytic disease of the newborn?

Answer 7

IgG

Question 8

Addition of LISS when performing an indirect antiglobulin test is designed to:

Answer 8

Lower the zeta potential of RBCs

Question 9

Phases of agglutination:

Answer 9

Sensitization –> lattice formation

Question 10

What is the optimum antigen:antibody ratio?

Answer 10

40: 1 (2 drops serum + 1 drop 5% RCS
133: 1 (4 drops serum + 1 drop 3% RCS)

Question 11

What is the anticoagulant used for AHG test?

Answer 11

EDTA

Question 12

What are the RBC morphology seen in acute and delayed HTRs?

Answer 12

Schistocytes; spherocytes