ABO & Rh Blood Group System

Question 1

Storage lesion or the biochemical changes in RBCs during storage will result in ____ plasma pH.

Answer 1

Decreased

Question 2

Property of cells wherein polyagglutination is a result of the exposure of a hidden antigen on RBCs due to bacterial or genetic infection

Answer 2

Tn activation

Question 3

Most helpful to confirm a weak ABO subgroup

Answer 3

Adsorption-elution

Question 4

What specific immunodominant sugar is responsible for group A antigenic specificity?

Answer 4

N-acetyl-D-galactosamine

Question 5

What is the predominant immunoglobulin class for anti-A and anti-B antibodies in group A and B individuals?

Answer 5

IgM

Question 6

Which of the Rh antigens has the highest frequency in Caucasians?

Answer 6

D

Question 7

Rh antibodies generally react best at ___C.

Answer 7

37

Question 8

ABO antigens are developed in utero at ____ of gestation; full expression occurs between ____ of age.

Answer 8

5-6 weeks; 2-4 years

Question 9

How many H antigen subtypes are there?

Answer 9

4

Question 10

List the genes according to their amount of H antigens (most to least)

Answer 10

O > A2 > A2B > A1 > A1B

Question 11

___% of random population are secretors.

Answer 11

78

Question 12

Detectable titers of ABO antibodies are seen within ____ and peaks at ____.

Answer 12

3-6 months; 5-10 years

Question 13

What is the principle used for determination of secretor status?

Answer 13

Hemagglutination inhibition

Question 14

What is the color of anti-A & anti-B reagent?

Answer 14

Blue; yellow

Question 15

What is the preservative used in the routine reagents used for ABO typing?

Answer 15

0.1% Na azide

Question 16

Anti-H lectin:

Answer 16

Ulex europaeus

Question 17

Anti-A1 (& anti-Tn, anti-Cad) lectin:

Answer 17

Dolichos biflorus

Helix pomatia

Question 18

Anti-B lectin:

Answer 18

Griffonia simplicifolia (Bandeiraea simplicifolia)

Question 19

Anti-T & anti-Tk lectin:

Answer 19

Arachis hypogaea

Question 20

Anti-T, anti-Tk & anti-Cad lectin:

Answer 20

Glycine soja

Question 21

Anti-Tn & anti-Tk lectin:

Answer 21

Salvia sclarea

Question 22

Anti-Tn & anti-Cad lectin:

Answer 22

Salvia hormium

Question 23

Anti-M lectin:

Answer 23

Iberis amara

Question 24

Anti-N lectin:

Answer 24

Vicia graminea & Bauhinia species

Question 25

Red cell antigen-antibody reactions serologic grading: several large aggregates, clear background

Answer 25

3+

Question 26

Red cell antigen-antibody reactions serologic grading: medium-sized agglutinates, clear background

Answer 26

2+

Question 27

Red cell antigen-antibody reactions serologic grading: small agglutinates, turbid background

Answer 27

1+