Basic Microbiology Laboratory Safety

Question 1

The term___________ is used to describe the
safe methods and procedures for handling and managing microorganisms in the laboratory.

Answer 1

“containment”

Question 2

It prevents workers from contaminating themselves with microorganisms, ensures that others and the work area do not become contaminated, and also ensures that microbial cultures do not become unnecessarily contaminated with unwanted organisms.

Answer 2

aseptic technique

Question 3

Kind of biosafety level which the agents not known to cause disease in
healthy adults; standard microbiological practices
(SMP) apply; no safety equipment required; sinks
required.

Answer 3

BSL 1

Question 4

• Agents associated with human disease;
  standard microbiological practices apply plus limited access, biohazard signs, sharps precautions, and a biosafety manual required.
• Biosafety
  cabinet (BSC) used for aerosol/splash generating operations; lab coats, gloves, face protection
  required; contaminated waste is autoclaved.

Answer 4

BSL 2

Question 5

—indigenous/exotic agents that may have serious or lethal consequences and with a potential for aerosol transmission

Answer 5

BSL 3

Question 6

Dangerous/exotic agents of a life threatening nature or unknown risk of transmission;

Answer 6

BSL 4