Basic knowledge CH 4 microscope

Question 1

Should not be used on lens

Answer 1

Xylene

Question 2

Measurement of the resolving power of a microscope

Answer 2

Numerical aperture

Question 3

Limit of usable magnification (Tell how small and how close individual objects can be and still be recognized)

Answer 3

Resolution

Question 4

10x lens and used for initial scanning for blood films and urine sediments, as an example.

Answer 4

Lower-Power lenses

Question 5

40x lens, used for detailed study such as histologic sections and wet preparation.

Answer 5

High power lenses

Question 6

100x, used for morphoric examination of blood films and microbes.

Answer 6

Oil-immersion lenses

Question 7

What is the function of the oil for the oil immersion lens?

Answer 7

The oil is used to increase the NA and thus the resolving power of the objective.

Question 8

Eyepiece; lens that magnifies the image formed by the objective

Answer 8

Ocular

Question 8

Primary image formation and play a central role in determining the quality of images

Answer 8

Objective lenses

Question 9

Controls the amount if light passing through the material under observation

Answer 9

Aperture

Question 10

Directs and focuses the beam of light from the bulb onto the material under examination.

Answer 10

Condenser

Question 11

Controls the amount of light shining up into the slide

Answer 11

Diaphragm

Question 12

If one objective is in focus and a change is made to another, the focus will not be lost

Answer 12

Parfocal

Question 13

The microscope is adjusted in such a way that the light path through the microscope, from the light source to the eye of the observer is correct.

Answer 13

Alignment

Question 14

What is the most common type of microscope used for general laboratory work?

Answer 14

Compound microscope (Bright field microscope)

Question 15

To magnify an object to a point where it can be seen with the human eye

Answer 15

Bright field microscope

Question 16

Uses scattered light to view objects that appear light on a dark background

Answer 16

Dark Field microscope

Question 17

Gives objects a three-dimensional appearence

Answer 17

Differential interference contrast microscope

Question 18

Automatically locate and reclassifies blood cells into categories. Digitizes images for electronic retrieval and sharing.

Answer 18

Digital microscope

Question 19

Provides greater magnification sharing and resolution than conventional light microscopy

Answer 19

Electron Microscope