Basic Concepts

Question 1

PCR

Question 2

What are adapter sequences?

Answer 2

contain the sequencing primer binding sites, the index sequences, and the sites that allow library fragments to attach to the flow cell lawn.

the sequencing primer anneals to the adapter, immediately upstream of the DNA insert

Question 3

NGS: Paired end vs Single end

Question 4

DNA Synthesis vs RNA Synthesis

Answer 4

DNA synth - semi conservative replication

RNA synth - transcription

Question 5

Promoter region, transcription factors

Answer 5

Bacterial: Promoters in bacteria contain two short DNA sequences located at the -10 (10 bp 5’ or upstream) and -35 positions from the transcription start site (TSS); the Pribnow box (TATAAT) is located at the -10 position and is essential for transcription initiation (EQUIVALENT TO TATA BOX IN EUKARYOTES)

Question 6

Types of mutations

Question 7

16S rrna, 50s 30s