Bacteriology quiz 1 Flashcards
It is an open fronted type of cabinet that uses an exhaust fan to allow
movement of air going through the open front
a. Class I
b. Class II
c. Class III
d. Class IV
a. Class I
It is appropriate for processing chemicals, radioisotopes and
carcinogens aside from biological samples treated with toxic or
hazardous chemicals. Airflow 0% recirculated and 100% exhausted.
a. Class I
b. Class III
c. Class II, Type A2
d. Class II, Type B2
d. Class II, Type B2
A pathogen that can cause human or animal disease but is unlikely to
be a serious hazard to laboratory workers, the community, livestock,
or the environment.
a. Risk Group 1
b. Risk Group 2
c. Risk Group 3
d. Risk Group 4
b. Risk Group 2
This is used in a gaseous form as a sterilant primarily in the
pharmaceutical and medical device manufacturing industries.
a. Hydrogen Peroxide
b. Peracetic Acid
c. Ethylene Oxide
d. None
b. Peracetic Acid
This is a diphenyl ether that disrupts the cell wall. The reaction time is intermediate, and the persistence is excellent. It has good activity
against gram-positive bacteria, gram-negative bacteria, and viruses.
a. Hexachlorophene
b. Chloroxylenol
c. Chlorhexidine gluconate
d. Triclosan
d. Triclosan
This is a saturated five-carbon dialdehyde that has broad spectrum activity and rapid killing action and remains active in the presence of organic matter.
a. Glutaraldehyde
b. Formaldehyde
c. Iodophors
d. None
a. Glutaraldehyde
Denature proteins; make lipids soluble
a. Halogens
b. Alcohols
c. Aldehydes
d. Phenolics
b. Alcohols
Disinfects and kills milk-borne pathogens and vegetative forms
a. Boiling water
b. Autoclave
c. Pasteurization
d. All of the above
c. Pasteurization
What is the pH indicator of Simmons’ citrate medium?
a. Phenol red
b. Trypticase nitrate
c. HCl
d. Bromothymol blue
d. Bromothymol blue
In DNase test medium what is added to the surface of the plate to precipitate unhydrolyzed DNA?
a. 1N HCl
b. 1 ml of xylene
c. both
d. neither
a. 1N HCl
Which of the following biochemical test uses p-dimethylaminobenzaldehyde (PDAB) as a reagent?
a. DNase
b. Indole
c. Citrate Utilization
d. All
b. Indole
This is a broth containing glucose; peptones; two pH indicators, bromocresol purple and cresol red; and the specific amino acid at a concentration of 1%. The medium has an initial pH of 6.0.
a. Simmons’ Citrate medium
b. Moeller decarboxylate base medium
c. Both
d. Neither
b. Moeller decarboxylate base medium
What is the purpose of α-naphthol in the Voges-Proskauer Test?
a. Maintains pH
b. pH indicator
c. Catalyst or color intensifier
d. All of the choices
c. Catalyst or color intensifier
Glucose is metabolized by the mixed acid fermentation pathway.
a. Voges-Proskauer Test
b. Malonate Utilization Test
c. Nitrite Reduction
d. Methyl Red test
d. Methyl Red test
What is the positive result in Malonate Utilization test?
a. Red
b. Yellow
c. Purple
d. Blue
d. Blue
What color in the Motility indole ornithine (MIO) agar indicates Ornithine decarboxylation?
a. Red
b. Yellow
c. Purple
d. Blue
c. Purple
What test uses N,N-dimethyl-α-naphthylamine and sulfanilic acid as reagents?
a. Nitrate reduction
b. Malonate utilization
c. Methyl Red Test
d. Motility Indole Ornithine
a. Nitrate Reduction
What test uses tetramethyl-ρ-phenylenediamine dihydrochloride as reagent?
a. Christensen’s urea
b. Kovac’s oxidase test
c. MIO
d. None
b. Kovac’s oxidase test
This test determines whether a microorganism can hydrolyze urea, releasing a sufficient amount of ammonia to produce a color change by a pH indicator.
a. Oxidase Test
b. Sulfide Indole Motility
c. Urease test
d. Nitrate Test
c. Urease Test
What is the enzyme being detected in the ONPG test?
a. Tryptophanase
b. Catalase
c. Cytochrome-c oxidase
d. β-Galactosidase
d. β-Galactosidase
This is for the presumptive identification of E. coli and Streptococcus anginosus group; enterohemorrhagic E. coli is negative
a. ONPG
b. PYR
c. MUG
d. LAP
c. MUG
In Nucleic acid hybridization test, what is referred to as the target strand?
a. Target
b. Template
c. Probe
d. Oligonucleotide
b. Template
This is used to detect RNA molecules, which are almost always transcript (messenger RNA, mRNA).
a. In solution hybridization
b. Southern Blot
c. Northern Blot
d. In situ hybridization
c. Northern Blot
A technique whereby chromosomal DNA could be separated by agarose gel electrophoresis and then transferred to and immobilized on a nitrocellulose membrane.
a. In solution hybridization
b. Southern Blot
c. Northern Blot
d. In situ hybridization
b. Southern Blot
In this hybridization method, DNA or RNA transcript can be detected directly in tissue with labeled probes. The technique is often performed directly in tissue that has been embedded in paraffin.
a. In solution hybridization
b. Southern Blot
c. Northern Blot
d. In situ hybridization
d. In situ hybridization
The goal of this step is to hybridize, oligonucleotide primers to the denatured, single-stranded target DNA strands.
a. Primer Annealing
b. Denaturation
c. Primer Extension
d. None
a. Primer Annealing
