Bacte quiz 2 Flashcards
What is test that provides a high probability for the presumptive identification of the β-hemolytic GAS and the nonhemolytic group D streptococci?
a. VP
b. CAMP
c. PYR
d. All
c. PYR
Described the three-dimensional structure of DNA molecule in the 1950s.
a. Hershey and Chase
b. Watson and Crick
c. Pauling and Franklin
d. Chargaff and Griffith
b. James Watson and Francis Crick
Columbia colistin-nalidix agar (CNA) is a selective medium for ____
a. Gram (-) organisms
b. Gram (+) organisms
c. Both
d. Neither
b. Gram (+) organisms
It’s a method by which genes are transferred or exchange between homologous regions on two DNA molecules.
a. Genetic recombination
b. Mutation
c. Crossing over
d. DNA Replication
a. Genetic Recombination
It was originally designed for US space exploration efforts of the 1970s as an onboard test system for spacecraft exploring other planets for life.
VITEK system
What is the most sensitive technique available for detecting and quantifying mRNA or transcript?
a. Northern Blot analysis
b. In situ hybridization
c. Reverse Transcription Polymerase Chain Reaction
d. None
c. Reverse Transcription Polymerase Chain Reaction
In this biosafety level examples are M. tuberculosis, St. Loius encephalitis virus, and Coxiella burnetii.
a. Biosafety level 1
b. Biosafety level 2
c. Biosafety level 3
d. Biosafety level 4
c. Biosafety level 3
There is a blood spill on a table top, what should be done properly?
a. Apply 70% alcohol and wipe with paper towel
b. Use a paper towel to allow the blood to be absorbed first, afterwards apply phenol and dry
c. Let it air dry and afterwards apply phenol and wipe with paper towel
d. None
b. Use a paper towel to allow the blood to be absorbed first, afterwards apply phenol and dry
A medtech intern was told to sterilize some materials and she noticed that they are heat sensitive. What is the best method she can perform?
a. Filtration method
b. Autoclave
c. Radiation
d. Dry heat
a. Filtration method
An organism are being observed are likely to cause life-threatening infection with no known modes of transmission. What BSL level should be used?
a. Biosafety Level 1
b. Biosafety Level 2
c. Biosafety Level 3
d. Biosafety Level 4
d. Biosafety Level 4
Group A β-hemolytic Streptococcus, for example, is not routinely tested because it is universally susceptible to penicillin, the drug of choice in treating infections caused by this bacterium.
a. True
b. False
a. True
These antibiotics act by binding to PBPs, bifunctional transglycosylases-transpeptidases responsible for the crosslinking of glycan strands and backbone peptide strands, respectively.
a. Group A β-hemolytic Streptococcus
b. β-Lactams
c. β-Lactamases
d. None
b. β-Lactams
These are antibiotics that affect DNA replication by targeting topoisomerases II (DNA gyrase) and IV.
a. Norfloxacin
b. Ciprofloxacin
c. Quinolones
d. All of the choices
c. Quinolones
In gram positive bacteria, the β-lactamases are localized to the periplasmic space, where they act on incoming β-lactam antibiotics.
a. True
b. False
b. False
Efflux pumps are found in which type of bacteria?
a. Gram positive
b. Gram negative
c. Gram positive and gram negative
c. Gram positive and gram negative
The gram-negative bacteria intrinsic resistance to vancomycin
a. True
b. False
a. True
This reversibly inhibits protein translation in bacteria by binding to the 30S ribosomal subunit and blocking entry of amino acyltRNA molecules into the A site of the ribosome, thus preventing the incorporation of amino acid residues into elongating peptide chains.
a. Tetracycline
b. Glycylcyclines
c. Ketolides
d. Tigecycline
d. Tigecycline
They bind close to the peptidyl transferase site of the 50S subunit of the bacterial ribosome inhibiting RNA-dependent protein synthesis.
a. Tetracycline
b. Glycylcyclines
c. Ketolides
d. Tigecycline
c. Ketolides
This antibiotic in combination with ciprofloxacin and clindamycin are reported to be useful for the treatment of bacterial infections relevant to biowarfare or bioterrorism, such as inhalational anthrax.
a. Rifamycins
b. Spectinomycin
c. Tetracycline
d. Macrolides
a. Rifamycins
This blocks the step leading to the formation of Tetrahydrofolate by preventing the dihydrofolate reductase–mediated recycling of folate coenzymes.
a. Sulfonamides
b. Trimethoprim
c. Folinic acid
d. Purines
b. Trimethoprim
A specific term referring to the property by which a biocide is able to inhibit bacterial multiplication; multiplication resumes upon removal of the agent.
a. Biocide
b. Bacteria
c. Bactericidal
d. Bacteriostatic
d. Bacteriostatic
Colonies are seen growing on the 3rd quadrant, what is the semi-quantitation grading?
a. Rare
b. Light
c. Moderate
d. Heavy
c. Moderate
In autoclaving, once the sterilization cycle is completed, molten agar is allowed to cool to approximately_______ before being distributed to individual petri plates.
a. 30C
b. 40C
c. 50C
d. 60C
c. 50C
This type of culture media contains growth enhancers that are added to nonselective agar to allow fastidious organisms to flourish.
a. Non selective
b. Selective Media
c. Differential Media
d. Enriched Media
d. Enriched Media
Gram stain of expectorated sputum reveals less than 25 white blood cells (WBCs) and more than 10 epithelial cells per low power field.
a. Accept the specimen
b. Reject the specimen
b. Reject the specimen
This process deposits cellular elements and microorganisms from the specimen onto the surface of a glass slide as a monolayer.
a. Smears from mucoid materials
b. Smear from thin fluids
c. Cytocentrifugation
d. None
c. Cytocentrifugation
