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Microbiology > Bacteriology morphology & agars > Flashcards

Bacteriology morphology & agars Flashcards

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Biology 101 flashcards
1
Q

Gram negative straight barrel shaped rods

A

Enterobactericeae

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2
Q

Gram + lancet shaped diplococci

A

Strep pneumo

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3
Q

Gram - kidney shaped diplococci

A

Neisseria

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4
Q

Gram + thick spore forming bacilli

A

Bacillus, Clostridia

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5
Q

Gram + small bacilli

A

Listeria

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6
Q

Gram +small bacilli in Chinese letters

A

Corynebacteria & Proprionibacteria (diptheroids)

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7
Q

Gram - curved rods

A

Vibrio

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8
Q

Gram - curved, S or corkscrew shaped rods, look like seagulls

A

Campylobacter

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9
Q

Gram - rods with safety pin appearance

A

Yersina pestis

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10
Q

Gram - coccobacilli in tissue

A

Legionella

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11
Q

Gram - highly pleomorphic bacilli when grown on BCYE: lollipop, dumbbell and teardrop shapes

A

Cardiobacterium hominis

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12
Q

Gram + branching, filamentous bacilli

A

Nocardia & Actinomyces

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13
Q

How often should blood cx’s be taken in suspected bacteremia? How much blood should be put in each bottle?

A

3 sets of 2 in a 24 hr pd, at least 30 minutes apart; >10mL

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14
Q

What are the steps for gram stain technique

A

Crystal violet, Gram iodine, Decolorize, Safranin, rinse

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15
Q

What dye is used in the acid fast stain and what does it bind to?

A

carbol fuscin, mycolic acid

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16
Q

How is the Kinyoun technique different from the Ziehl-Neelsen? Fite?

A

The kinyoun is a cold technique while Ziehl is a hot technique using a stronger 3% HCl; In Fite a weaker 1% H2SO4 is used **Nocardia and M leprae may be acid-fast with Fite and not Ziehl

17
Q

What are the AFB organisms?

A

Mycobacteria, Nocardia, Cryptosporidium, Microsporidium, Isospora, Sacrocystis, Legionella micdadei, Rhodococcus equi, Saccharomyces

18
Q

Campy-BAP

A

selects for Campylobacter

19
Q

Buffered charcoal yeast extract (BCYE)

A

Legionella; agar with antibiotics will inhibit most other upper respiratory flora and leave Legionella growing

20
Q

Thayer-Martin agar

A

Neisseria spp

21
Q

MacConkey agar

A

Selects for gram negative bacteria (bile salts) and differentiates between fermenting/non with lactose

22
Q

Red colonies on MacConkey

A

Escherichia, Klebsiella, Enterobacter (strong lactose fermenters)

23
Q

Pink colonies on MacConkey

A

Citrobacter, Providencia, Serratia, Hafnia

24
Q

Colorless colonies on MacConkey

A

Proteus, Edswardsiella, Salmonella, Shigella

25
Q

Eosin methylene blue agar (EMB)

A

selects for gram negative bacteria (aniline dye) and differential media

26
Q

Green-black-purple on EMB agar

A

Escherichia, Klebsiella, Enterobacter, Serratia, Hafnia, Citrobacter, Providencia

27
Q

Colorless colonies on EMB agar

A

Proteus, Edwardsiella, Salmonella, Shigella

28
Q

Salmonella-Shigella agar

A

Inhibits growth of most coliforms (bile sals and sodium citrate) and has lactose

29
Q

Colorless colonies on S-S agar

A

Shigella

30
Q

Colorless colonies with black center on S-S agar

A

Salmonella

31
Q

Hektoen-enteric agar (HE)

A

same purpose as S-S agar

32
Q

Salmon-pink to orange colonies on HE agar

A

fermenters (E.coli)

33
Q

Green colonies on HE agar

A

Shigella

34
Q

Green colonies with black center on HE agar

A

Salmonella

35
Q

Mannitol salt agar (MSA)

A

inhibits most gram-negative organisms. Can be used to isolate Staph aureus from mixed cultures

36
Q

At what temperature is Listeria most motile? Is that the only temperature it grows at?

A

tumbling motility only at 25 degrees C

Can also grow at 37 degrees C

37
Q

What two species grow at 25 to 30 degrees C?

A

Pseudomonas fluorescens and Mycobacterium marinum

38
Q

What is the optimal temperature for initial plating of most organisms?

A

37 degrees C

Microbiology (5 decks)

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