Bacteriology EC Flashcards
Peptidoglycan Function
Gives rigid support
Protects against osmotic pressure
(What stains on gram stain)
Major surface antigen of Gram+
Lipoteichoic acid (induces TNF/IL-1)
Cell wall (made of peptidoglycan)
Major surface antigen of Gram -
LPS/Endotoxin/Outer membrane
Lipid A induces TNF/IL-1
O polysaccharide is the anitgen
What is the periplasm?
Space between cytoplasmic membrane and outer membrane in gram -
Contains BETA-LACTAMASE
How do beta-lactams work?
Inhibit cross-linking of peptidoglycan by transpeptidase
Gram + Bacteria (gram stain color, major antigen, vulnerability to beta-lactams)
BLUE
Thick cell wall/peptidoglycan (+ is thick)
Lipoteichoic acid
Vulnerable to beta-lactams/lysozymes
Gram - Bacteria (gram stain color, major antigen, vulnerability to beta-lactams)
RED
Thin cell wall/peptidoglycan (- is thin)
LPS (lipid A) Endotoxin
Resistant to beta lactams/lysozymes (beta-lactamase in periplasmic space)
What are the gram + bacteria?
Mycobacterium (acid fast) Gardnerella Staph Strep Corynebacterium Actinomyces Nocardia Bacillus Listeria Clostridium
“My God, Some Silly Corny Actors kNock Back Listerine in the Closet”
What Gram + bacteria commonly cause disease and what is their morphology?
Streptococcus Enterococcus Staphylococcus Bacillus (spore forming) Clostridium (spore forming) Corynebacterium (rods) Listeria (rods)
What are the morphologies of the Gram - bacteria?
Neisseria (diplococcus)
Treponema pallidum (spirochete)
REST=rods/pleomorphic
What stain identifies mycobacterium?
Acid fast (causes TB and leprosy)
What bacteria have unusual cell membranes/walls?
Mycoplasma - STEROLS and NO CELL WALL
Mycobacterium - MYCOLIC ACID and HIGH LIPID CONTENT
What bacteria do not Gram stain well?
Treponema (too thin- use darkfield microscope)
Rickettisa (intracellular)
Mycobacteria (lipid content detected in acid fast stain
Mycoplasma (no cell wall)
Legionella (intracellular)
Chlamydia (intracellular)
“These Rascals May Microscopically Lack Color”
What bugs stain with Giemsa?
Chlamydia Borrelia Rickettsiae Trypanosome Plasmodium
“Certain Bugs Really Try my Patience”
What bugs stain with PAS (periodic acid-Schiff)?
Tropheryma whipplei
(Stains glycogen)
“PAS the sugar”
What bugs stain with Ziehl-Neelsen?
Acid fast
Nocardia
Mycobacterium
What bugs stain with India Ink?
Cryptococcus neoformans
What bugs stain with Silver stain?
Fungi (ie Pneumocystis)
Legionella
H. pylori
What does catalase do?
Breaks down hydrogen peroxide radicals
What does peroxidase do?
Breaks down hydrogen peroxide radicals
What does superoxide dismutase do?
Breaks down superoxide radicals
What are obligate aerobes?
Must use oxygen - same enzymes as us
What are the obligate aerobes?
Nocardia
Pseudomonas
Mycobacterium TB
Bacillus
“Nagging Pests Must Breathe”
What are facultative anaerobes?
Have the faculty to be anaerobic (comparable to skeletal muscle during sprinting)
Prefer O2, but switch if have to
Are obligate anaerobes?
Hate oxygen (no enzymes to defend against it)
What are the obligate anaerobes?
Clostridium
Bacteroides
Actinomyces
“Can’t Breathe Air”
AninOglycosides are ineffective because they require O to enter cell
What bugs are obligate intracellular?
Rickettsia
Chlamydia
(can’t make own ATP)
“stay inside when it gets Really Cold”
What bugs are facultative intracellular?
Salmonella Neisseria Brucella Mycobacterium Listeria Francisella Legionella Yersinia
“Some Nasty Bugs May Live FacultativeLY”
What does the Quellung reaction test for?
Encapsulated bacteria
“Quellung=Swelling”
What bacteria are encapsulated?
E. coli Strep. pneumoniae Klebsiella pneumoniae H. influenzae Neisseria meningitidis Salmonella B Strep
“Even Some Killers Have Nice Shiny Bodies”
What organisms would someone with chronic granulomatous disease get recurrent infections from? Why?
Catalase + organisms Pseudomonas Listeria Aspergillus Candida E. coli S. aureus Serratia
“you need PLACESS for your CATs”
People with CGD have a NADPH oxidase deficiency
Catalase + bacteria neutralize limited H2O2 produced
What bugs are urease +?
Cryptococcus H. pylori Proteus Ureaplasma Nocardia Klebsiella S. epidermidis S. saprophyticus
“CHuck norris hates PUNKSS”
What bacteria produce pigment?
Actinomyces ISRAELii - yellow sulfur granules “Israel has yellow sand”
S. aureus - yellow pigment “aureus=gold’
Pseudomonas AURUGinosa - blue-GREEN pigment “AERUGala is GREEN”
Serratia MARCESCENS - RED pigment “red maraschino cherries”
Corynebacterium diphtheriae (Toxin, Mechanism, Manifestation)
Diptheria toxin
Inactivates EF-2 (elongation factor)
Pseudomembranous pharyngitis Severe lymphadenopathy ("bull neck")
Pseudomonas aeruginosa (Toxin, Mechanism, Manifestation)
Exotoxin A
Inactivates EF-2 (elongation factor)
Host cell death
Shigella (Toxin, Mechanism, Manifestation)
Shiga toxin
Inactivates 60S ribosome (remove adenine from rRNA)
GI mucosal damage –> dysentery
Cytokine release –> HUS
EHEC O157:H7 (enterohemorrhagic E. coli) (Toxin, Mechanism, Manifestation)
Shiga Like Toxin
Inactivates 60S ribosome (remove adenine from rRNA)
Enhances cytokine release –> HUS
Does no invade host cells
ETEC (enterotoxigenic E. coli) (Toxin, Mechanism, Manifestation)
Heat-LABILE (LT) - overactivates adenylate cyclase, increase cAMP, increase CL and water secretion
Heat-STABLE - overactivates guanylate cyclase, increased cGMP, decreased NaCl and H2O resorption
Watery diarrhea results
“Labile in the Air (Adenylate cyclase)
Stable on the Ground (Guanylate cyclase)”
Bacillus anthracis (Toxin, Mechanism, Manifestation)
Edema factor
Mimics adenylate cyclase (increased cAMP)
Edematous borders of black eschar in cutaneous anthrax
Vibrio cholerae (Toxin, Mechanism, Manifestation)
Cholera toxin
Permanently activates Gs (increased cAMP)
Cl secretion and H2O efflux
“Rice water” diarrhea
Bordetella pertussis (Toxin, Mechanism, Manifestation)
Pertussis toxin
Disables Gi (increased cAMP) Inhibits phagocytosis
Whooping cough
Clostridium tetani (Toxin, Mechanism, Manifestation)
Tatanospasmin
Cleave SNARE and prevent release of GABA/glutamate
Spastic paralysis “Lock jaw”
Clostridium botulinum (Toxin, Mechanism, Manifestation)
Botulinum toxin
Cleave SNARE and prevent release of ACh
Flaccid paralysis “Floppy baby”