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Bacteriology > Bacteriology 3 > Flashcards

Bacteriology 3 Flashcards

1
Q

What does the choice of diagnostic method depend on?

A

The type of infection and availability of tests

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2
Q

What are different ways to identify the agent?

A

Direct detection - staining
Isolation
Direct detection of antigens, toxins
PCR

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3
Q

What are different ways to identify the host immune response?

A

Humoral immune response

Cell mediated immune response

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4
Q

What are biosafety levels (BSL)?

A

Used to minimize potential exposure to a biohazard for lab personnel and others.

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5
Q

What happens when the BSL increases?

A

The risk of the organism to humans, facility requirements for working with organism also increases.

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6
Q

What is the single most important and cost effective
lab procedure which will provide
immediate info on the # and morphologic characteristics of microorganisms?

A

Light microscopy

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7
Q

What are some types of culture media?

A

Liquid Broth media, solid agar media, selective media

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8
Q

What does capnophilic culture need?

A

Carbon dioxide

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9
Q

What are some methods of bacterial identification?

A 
Biochemical (kits/automated systems)
Antigenic (sero-grouping/serotyping)
Phage typing
Virulence typing
Whole-cell fatty acid analysis
Genetic analysis
Protein analysis
Enzyme iso-typing
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10
Q

What type of test measures proteins produced by bacteria, and is often used in research?

A

MALDI-TOF mass spectrometry

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11
Q

For PCR, what is the ultimate goal?

A

Amplify the gene or groups of genes specific to that bacteria

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12
Q

What are the components of a PCR reaction?

A

Primers, nucleotides, enzyme Taq polymerase, target DNA

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13
Q

What are some tests used to detect the presence of the antigen?

A

ELISA, agglutination, Fluorescent antibody staining

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14
Q

What are some tests used to detect the presence of chemicals?

A

Chromatography, mass spectrometry

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15
Q

What are some direct tests for biological detection?

A

Presence of LPS

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16
Q

How can one detect a specific immune response to the bacteria?

A

Agglutination, precipitation, ELISA, cell medicated immune response, tuberculin rxn, interferon gamma

17
Q

Which antibody is seen with 1st contact, or primary immune response?

A

IgM

18
Q

Which antibody is seen with 2nd contact, or secondary immune response?

A

IgG

19
Q

T/F: Primary immune response is faster than secondary.

A

False, primary is slower, it takes some time to see antibodies

20
Q

How can we measure the amount of antibodies produced during disease?

A

Antibody titer

21
Q

T/F: The higher the antibody response the higher the titer.

A

True

22
Q

What is antibody development after exposure to a pathogen or an antigen?

A

Seroconversion

23
Q

What are 2 examples of cell mediated immune responses?

A

TB test & interferon gamma test

Bacteriology (5 decks)

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