Bacteriology 3 Flashcards
What does the choice of diagnostic method depend on?
The type of infection and availability of tests
What are different ways to identify the agent?
Direct detection - staining
Isolation
Direct detection of antigens, toxins
PCR
What are different ways to identify the host immune response?
Humoral immune response
Cell mediated immune response
What are biosafety levels (BSL)?
Used to minimize potential exposure to a biohazard for lab personnel and others.
What happens when the BSL increases?
The risk of the organism to humans, facility requirements for working with organism also increases.
What is the single most important and cost effective
lab procedure which will provide
immediate info on the # and morphologic characteristics of microorganisms?
Light microscopy
What are some types of culture media?
Liquid Broth media, solid agar media, selective media
What does capnophilic culture need?
Carbon dioxide
What are some methods of bacterial identification?
Biochemical (kits/automated systems) Antigenic (sero-grouping/serotyping) Phage typing Virulence typing Whole-cell fatty acid analysis Genetic analysis Protein analysis Enzyme iso-typing
What type of test measures proteins produced by bacteria, and is often used in research?
MALDI-TOF mass spectrometry
For PCR, what is the ultimate goal?
Amplify the gene or groups of genes specific to that bacteria
What are the components of a PCR reaction?
Primers, nucleotides, enzyme Taq polymerase, target DNA
What are some tests used to detect the presence of the antigen?
ELISA, agglutination, Fluorescent antibody staining
What are some tests used to detect the presence of chemicals?
Chromatography, mass spectrometry
What are some direct tests for biological detection?
Presence of LPS
How can one detect a specific immune response to the bacteria?
Agglutination, precipitation, ELISA, cell medicated immune response, tuberculin rxn, interferon gamma
Which antibody is seen with 1st contact, or primary immune response?
IgM
Which antibody is seen with 2nd contact, or secondary immune response?
IgG
T/F: Primary immune response is faster than secondary.
False, primary is slower, it takes some time to see antibodies
How can we measure the amount of antibodies produced during disease?
Antibody titer
T/F: The higher the antibody response the higher the titer.
True
What is antibody development after exposure to a pathogen or an antigen?
Seroconversion
What are 2 examples of cell mediated immune responses?
TB test & interferon gamma test