Bacterial Replication Flashcards

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1
Q

What is the cell cycle?

A

A sequence of events from the formation of the new cell through the next cell division.

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2
Q

How do bacteria replicate?

A

Binary fission.

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3
Q

What are the three phases of the bacterial cell cycle?

A
  1. Period of growth after the cell is born.
  2. Chromosome replication and partitioning.
  3. Cytokinesis (septation).
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4
Q

What occurs during the first step of binary fission?

A

a. The cell prepares for division by enlarging its cell wall.
b. Plasma membrane enlarges.
c. Overall volume increases.
d. DNA replication begins.

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5
Q

What occurs during step 2 of binary fission?

A

a. Septum development begins.
b. Chromosomes move to opposite ends of the connected, developing cells.
c. Other cytoplasmic components are evenly distributed throughout the two developing cells.

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6
Q

What occurs during step 3 of binary fission?

A

The septum is synthesized completely through the cell center, creating two separate cell chambers.

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7
Q

What occurs during step 4 of binary fission?

A

The daughter cells are separate or, in some species, remain attached in a chain.
*Some bacterial cells will begin binary fission before they are finished dividing, allowing for more rapid multiplication.

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8
Q

How many origins of replication are there on each chromosome in a prokaryotic cell?

A

1.

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9
Q

What enzyme is used to synthesize DNA in prokaryotic cells?

A

DNA Pol. III.

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10
Q

What enzyme is required for the removal of RNA primers and replacement of RNA with DNA?

A

DNA polymerase I.

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11
Q

What is septation?

A

The formation of a cross wall between two daughter cells.

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12
Q

What is step 1 of septation?

A

Selection of site for septum formation.

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13
Q

What is step 2 of septation?

A

Assembly of Z ring from FtsZ proteins after the replication of genetic material.

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14
Q

What is step 3 of septation?

A

Assembly of cell wall-synthesizing machinery.

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15
Q

What is step 4 of septation?

A

Constriction of cell and septum formation.

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16
Q

What occurs during the cytoplasmic stage?

A

Synthesis of precursors (NAG and NAM).

17
Q

What attaches to the NAM?

A

Pentapeptides.

18
Q

What occurs during the membrane stage?

A

Transfer of precursors from cytosol to membrane and incorporation into growing peptidoglycan.

19
Q

What enzyme is present in the membrane stage?

A

Peptidoglycan synthase.

20
Q

What is peptidoglycan synthase?

A

An enzyme that transports bactoprenol across the cell wall.

21
Q

What protein is used during the membrane stage?

A

Bactoprenol.

22
Q

What is bactoprenol?

A

A protein that binds the NAG-NAM complex for transport across the membrane.

23
Q

What role does phosphatase have in the membrane stage?

A

It dephosphorylates bactoprenol, allowing it to be recycled back into the cytoplasm.

24
Q

What happens during the extracellular stage?

A

Crosslinking of linear chains of peptidoglycans by membrane bound transpeptidases.

25
Q

What is antibiotic targets transpeptidase?

A

Penicillin, making it a penicillin binding protein (PBP).

26
Q

What are most of the enzymes and proteins used in peptidoglycan wall synthesis used as?

A

Drug targets.