Bacterial Genetics

Question 1

Name some examples of toxins that were acquired by phages?

Answer 1

COBEDS
C- cholera
O - o ag of salmonella
B - botulinum
E - erythrogenic toxin of s pyogenes
D - diptheria toxin
S - shiga toxin

Question 2

What does homologous recombination absolutely require in order to occur?

Answer 2

Rec A expression

Question 3

Name three bacteria that are competent for transformation?

Answer 3

1. s pneumoniae
2. H flu
3. Neisseria
   (the bacteria that have IgA protease)

Question 4

Is homologous recombination required in:

1. transformation
2. conjugation
3. transduction

Answer 4

1. yes
2. only for HFR x F -
3. yes

Question 5

In HFR conjugation, genes are most likely transferred if they are located next to _____

Answer 5

OriT

Question 6

Differentiate btwn generalized and specialized transduction

Answer 6

generalized - “packaging event”

specialized “an excision event”