Bacterial Cultures

Question 1

Chocolate agar with factors V (NAD+) and X (Hematin) is used to isolate ______?

Answer 1

H. influenzae

Question 2

Thayer Martin (or VPN) Media: Composition?
(Hint: There is a mnemonic)

Answer 2

Thayer Martin: VPN Media
V = Vancomycin –> will (-) Gram (+) organisms
P = Polymixins –> (-) Gram (-) EXCEPT Neisseria
N = Nystatin –> (-) Fungi

Mnemonic:
“To connect to Neisseria, please user your VPN client”

Question 3

Bordet-Gengou (potato) Agar

Answer 3

Bordatella

Thing “Bordet for Bordatella”

Question 4

Tellurite plate, Loffler’s Media

Answer 4

C. diptheriae

Question 5

Lowenstein - Jensen agar

Answer 5

M. TB

Question 6

Eaton’s Agar

Answer 6

M. Pneumoniae

Question 7

MacConkey’s Agar: What is it?

Answer 7

An agar with lactose: fermentation produces acid, which turns the colony pink

Question 8

What is MacConkey Agar used to isolate?

Answer 8

lactose Fermenting Enterics

Question 9

Eosin-Methylene Blue Agar - used for what?

Answer 9

E. coli

colonies grow with a green metallic sheen

Question 10

Charcoal Yeast extract agar buffered with cysteine and iron

Answer 10

Legionella

Question 11

Sabouraud’s Agar

Hint: there is a mnemonic

Answer 11

Fungi

“Sab’s a Fun Guy”