Bacterial culture media

Question 1

Chocolate agar

Answer 1

H. influenzae

Contains factors V and X (NAD+ and hematin, not coag factors)

Question 2

Thayer-Martin agar

Answer 2

Neisseria sp
Contains vancomycin (G+), Trimethoprim, Colistin (other G-), Nystatin (fungi) for selectively isolating Neisseria

Question 3

Bordet-Gengou agar

or Regan-Lowe medium

Answer 3

Bordetella pertussis
Contains potato
or charcoal, blood, antibiotic

Question 4

Tellurite agar, Loffler medium

Answer 4

C. diphtheriae

Question 5

Lowenstein-Jensen agar

Answer 5

M. tuberculosis

Question 6

Eaton agar

Answer 6

M. pneumoniae

Question 7

MacConkey agar

Answer 7

For [lactose-fermenting] enterics (E. coli, Klebsiella)
Contains bile to block G+
If a fermenter -> produces acid -> colonies turn pink
Otherwise -> colonies turn white
Differential media (not selective)

Question 8

Eosin-methylene blue (EMB) agar

Answer 8

E. coli

Colonies have green metallic sheen

Question 9

Charcoal yeast extract agar w/ Cys and Fe

Answer 9

Legionella

Question 10

Sabouraud agar

Answer 10

Fungi