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Bacteriology > Bacterial Cultivation > Flashcards

Bacterial Cultivation Flashcards

1
Q

Refers to the increase in the number of bacteria rather than in size.

A

BACTERIAL GROWTH

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2
Q

True or False

In bacteria, growth refers to the number, not in size

A

TRUE

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3
Q

Bacterial growth requirements

A

NUTRITIONAL & ENVIRONMENTAL

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4
Q

Salt-loving bacteria

A

HALOPHILIC/HALOPHILES

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5
Q

Halophilic bacteria

A

Staphylococcus spp.
Enterococcus spp.
Vibrio spp. except Vibrio cholerae and Vibrio mimicus
Bacillus spp.

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6
Q

Bacteria that are difficult to grow

A

FASTIDIOUS

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7
Q

Absolutely requires oxygen to grow l, live, and survive

A

STRICT/OBLIGATE AEROBE

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8
Q

Strict/Obligate Aerobe

A

Micrococcus spp.
Mycobacterium spp.
Pseudomonas spp.
Neiserria spp.
Brucella spp.
Francisella spp.
Bordatella spp.
Leptosprira spp.

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9
Q

Bacteria that have the ability to grow, live, and survive in small concentration of oxygen environment( hindi maarte)

A

FACULTATIVE ANAEROBE

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10
Q

Facultative Anaerobe

A

Staphylococcus spp.
Streptococcus spp.
Family of Enterobacteriaceae

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11
Q

Bacteria that prefers small concentration of oxygen environment approximately 2%-10%

A

MICROAEROPHILIC

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12
Q

Microaerophilic

A

Campylobacter spp.
Helicobacter spp.
Arcobacter spp.
Streptococcus spp.

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13
Q

Absolutely do not require oxygen to grow, live, and survive.

A

STRICT/OBLIGATE ANAEROBE

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14
Q

Strict/Obligate Anaerobe

A

Many Clostridium spp., such as Clostridium noyvi, most Bacteroides spp., Fusobacterium spp., Peptostreptococcus spp., and Porphyromonas spp.

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15
Q

Bacteria that do not require oxygen but may tolerate or withstand limited exposure to oxygen.

A

AEROTOLERANT ANAEROBE

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16
Q

Aerotolerant Anaerobe

A

Some Clostridium spp., such as Clostridium perfringens, Bacteroides fragilis, most strains of Proprionibacterium and Lactobacillus

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17
Q

Bacteria that requires 5%-10% CO2 to grow

A

CAPNOPHILIC/ CAPNOPHILES/
CAPNOPHILIC BACTERI

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18
Q

Capnophilic Bacteria

A

(N) Neisseria spp. (C) Cardiobacterium spp.
(H) Haemophilus spp. (E) Eikenella spp.
(A) Aggregatibacter spp. (K) Kingella spp.

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19
Q

Optimally growing between 20-40˚C

A

MESOPHILIC

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20
Q

Growing optimally between 0-20˚C

A

PSYCHROPHILIC

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21
Q

50-60˚C

A

THERMOPHILIC

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22
Q

80-110˚C & Spore-forming bacteria

A

HYPERTHERMOPHILIC

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23
Q

Wide range of temperature

A

EURITHERMOPHILIC

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24
Q

Wide range of temperature

A

STERNOPHILIC

25
Q

Acid loving bacteria

A

ACIDOPHILIC

26
Q

Alkali loving bacteria

A

ALKALIPHILIC

27
Q

High osmotic pressure

A

OSMOPHILIC BACTERIA

28
Q

The bacteria are adjusting to the environment
Cellular activity: synthesis of proteins & DNA is already happening. No cell division is happening
* The cell activities are needed for the cell division

A

LAG PHASE

29
Q
  • From the lag phase, the logarithmic suddenly rises; the bacteria are increasing; rapid generation of bacteria.
  • Growth rate: The number of generation per hour
  • The phase where bacteria is the most metabolically active; susceptible to antimicrobial agents
  • There is cell division = increase in number
A

LOGARITHMIC GROWTH PHASE

30
Q
  • The nutrients are running out; toxins are produced that are harmful to microorganisms
  • Rate of cell division = Rate of cell deaths
A

STATIONARY OR PLATEU PHASE

31
Q

Bacterial growth no longer occurs; more dying than viable

A

DEATH (DECLINE PHASE)

32
Q

Mimicking the environment and nutrition that
is originally the reservoir

A

CULTURE MEDIA

33
Q

Composed of known and exact amounts of pure chemical substances; commonly used for culturing autotrophic microorganisms

A

SYNTHETIC/CHEMIMCALLY DEFINED

34
Q
  • Composed of complex materials that are rich in vitamins and nutrients that are not usually represented by a chemical formula such as peptones, beef or yeast extract, plant extracts etc.
  • Combination of chemicals and complex materials that are rich in minerals and vitamins
A

NON-SYNTHETIC/NON-CHEMICALLY DEFINED/ COMPLEX

35
Q
  • Live cells harvested from organs of humans and animals that supports the growth of obligate intracellular organisms that
    cannot grow in artificially prepared culture media.
  • Commonly used for viruses
A

TISSUE CULTURE MEDIA

36
Q

a culture medium that doesn’t contain a solidifying agent; dissolved in water

A

LIQUID

37
Q
  • A culture medium that contains 0.5% - 1% agar
  • Solidifying agent: agar (most common) (it is not easily degraded by bacteria; it can be melted and can be resolidify upon cooling: 42˚C then melt upon boiling)
  • Sulfide Indole Motility (SIM) Medium: Used for observation of hydrogen sulfide gas production, indole production & motility
  • What is often desired to be observed is motility
A

SEMI-SOLID

38
Q

A culture medium which contains 1.5% - 3%T (2%-3% in other books) agar

A

SOLID

39
Q

Usually contained in a container that can be made of glass (pyrex) or disposable plastic (standard: 100 mm in terms of diameter) (AST: 150mm)

A

PLATED

40
Q

Usually, container in glass tubes such as Wassermann tubes with different volume capacity (3mL, 5mL, 10mL) or in a tube
with a flat bottom and a screw cap

A

TUBED

41
Q

Culture media contained in a glass bottle that is usually used for blood culture

A

BOTTLED

42
Q
  • Contains basic nutritional requirements to support the growth of non-fastidious microorganisms
  • This is also used a base medium in the preparation of other culture media
A

GENERAL PURPOSE/ PRIMARY/
BASIC/ BASAL/ SUPPORTIVE/
GENERAL ISOLATION CULTURE MEDIA

43
Q

Contains the basic nutritional requirement to support the growth of non-fastidious microorganisms with additives (special requirements: blood), enriching substances, or supplements to support the growth of fastidious microorganisms. Ex: BAP, CAP

A

ENRICHED CULTURE MEDIA

44
Q

A primary media used to support or favor the selective growth of pathogens in a specimen, such as stool or sputum, where the number of normal flora outnumber the pathogens

A

ENRICHMENT BROTH

45
Q

Favors the growth of the organism of interest using inhibitors added in the culture media

A

SELECTIVE CULTURE MEDIA

46
Q

Contains indicators which changes in color as a result of a product produced be a chemical reaction in the components of the media such as glucose

A

DIFFERENTIAL CULTURE MEDIA

47
Q

True or False

Not all selective culture media are differential but all differential culture media are selective

A

TRUE

48
Q

A primary isolation culture media which maintains the viability of bacteria allowing rapid multiplication if there is an anticipated delay in bringing the specimen collected bedside or remotely to the laboratory

A

TRANSPORT CULTURE MEDIA

49
Q

Stuart’s Transport Medium

A

Neisseria spp.

50
Q

Cary-Blair

A

Vibrio spp.

51
Q

Amie’s

A

Neisseria spp. (with charcoal)

52
Q

Transgrow, JEMBEK

A

brand

53
Q

JEMBEK

A

Jon E. Martin Biological, Environmental, Chamber

54
Q

Used to selectively favor the growth of Vibrio while inhibiting all other normal intestinal flora due its high Ph

A

Alkaline Peptone Water (APW)

55
Q

Used to selectively favor the growth of Salmonella while inhibiting all other normal intestinal flora

A

Selenite F Broth

56
Q

An enrichment broth for anaerobic bacteria but should be used solely in the isolation
of anaerobic bacteria since it can also grow aerobes and facultative anaerobes

A

Thioglycolate Broth (THIO)

57
Q

Used to selectively favor the growth of Salmonella and Shigella while inhibiting all other normal intestinal flora

A

GN Broth (Gram Negative Broth)

58
Q

A liquid enrichment recommended for the
production of Streptococcal haemolysin (virulence factor of Streptococcus) and the cultivation of Streptococci prior to
serological grouping

A

Todd-Hewitt Broth

59
Q
A

Bacteriology (2 decks)

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