Bacteria Cocci Gram - Flashcards
Gram - Diplococci
Oxidase +
Neisseria Species
Easily spread in areas with a lot of people, military recruits, college dorms via respiratory droplets.
Gram - Diplococci Oxidase +
Maltose Fermenter
Neisseria Meningitidis
Diference between N. Meningitidis
and N. Gonorrhea
N. Meningitidis can ferment Maltose from glucose
Nesseria’s Species Agars
Chocolate Agar
VPN (vancomycin, polymixin, and nystatin) Agar or THYLER MARTIN
MAC Deficiency unable to form the MAC complex due to complex c5-c9 being inhibited
complex c5-c9 being inhibited
Compliment deficiency predisposes to unable to form the MAC complex for encapsulated organisms.
C5-C9 compliment
- Sickle cell disease are more susceptible to Neisseria meningitides because it is encapsulated (ASPLENIA)
Neisseria Species Virulence Factors
- Pilli allows attachment to surfaces and display antigenic variation
- IgA protease will cleave IGA at its hinge point, facilitates survival along mucosal surfaces .
Nasopharyngeal swab suggests that it is found in nasal cavities first and transmitted via
N. Meningitides
Polysaccharide capsules incluides in N. Meningitides Vaccine
A.C.D NOT B
common serotype of N. Meningitides
B
Major virulence factor N. Meningitides
Capsule is the major virulence factor, type B strain is not included in the vaccine
Responsible for the inflammatory cascade N. Meningtides
lipooligiosaccharides
N. Meningitidis Pathogenesis
LOS (Inflammatory response) -> Leakage of interstitial fluid by capillaries) hypovolemia (Petiquias) -> vasoconstriccion (leads to Waterhouse- Fredd Sydrome-> DIC -> SHOCK.
N. Meningitidis infection leads to
- DIC
- Waterhouse-Fredd Syndrome (necrosis hemorrágica suprarrenal)
mortality rate N. Meningitidis
15%
Treatment to N, Meningitidis infection
Cextriazone
Close contacts Treatment to N, Meningitidis infection
Rifampicin
Sexually transmitted infection Nesseria
N. Gonorrhea
Facultative intracellular and invade PMN’s Neiserria
N. Gonorrhea
Not encapsulated Neisseria
N. Gonorrhea
N. Gonorrhea in male’s causes
Urethritis and prostatitis and orchiditis
Female infection N. Gonorrhea
-Pelvic inflammatory Disease - purulent white discharge
Pelvic inflammatory Disease complication
- PID spreading to peritoneum - Fitz Hugh Curtiss syndrome
“Violin string” like adhesions in liver from spread into peritoneum
Fitz Hugh Curtiss syndrome (PID spreading to peritoneum)
May cause asymmetric polyarthritis in the knee
N. Gonorrhea
Cause a conjunctivitis w/ in 5 days of birth
N.Gonorrhea
We asume a px infected with N. Gonorrhea is also infected by?
Chlamydia Trachomatis
.TXT for N. Gonorrhea infection
Cextriazone + Azitromicin (chlamydia)
Gram - Bacilli
Bordetella Pertussis Haemophilus Influenzae Legionella Brucella Francisella Telarensis Coxiella burnetii Pastuerella Multocidida Bartonella henselae
Zoonotic Infections
Brucella Francisella Telarensis Coxiella burnetii Pastuerella Multocidida Bartonella henselae
Pilus called filamentous hemagglutini
Bordetella Pertussis
Pertussis Toxins
- ADP (adenylate cyclase)
- Tracheal Toxin
Pertussis Toxins functions
Tracheal toxin: damages ciliated cells in the epithelium, tractor cuts long cilia grass.
-Adenylate cyclase toxin:acts like the anthracis toxin edema factor, increases cAMP, Edema Factor, Most Virulent
Pertusis Stages
- Catarrhal: 2weeks of symtoms from upper resp infection.
- Parosxysms coughing; Whoop!
- Convalescent stage:subsiding cough.
Characteristic Pertusis Cough
Whooping sound
Parosxysms Coughing
Convalescence stage on Pertusis
final stage lasting 3 months with a cough, 100 day cough, most susceptible to secondary infections
TXT Bordetella Pertusis
Macrolides
Pertusis vaccine
DTaP - acellular vaccine using purified antigens
Aerobic,Non motile,Gram Neg rod
Bordetella Pertussis
Grown on chocolate agar needs factor 5 (NAD, nicotinamide) and factor 10 (Hemodin) “hemoTEN”
Haemophilus Influenz
Gram -,Coccobacillary, Grown in chocolate agar
Haemophilus Influenza
Need factors on agar for Haemophilus Influenza grow
Factor 5; Nicotinamide
Factor 10: hemotin
ON chocolate agar.
Haemophilus Influenza causes:
- Pneumonia: by aerosol transmission
- Epiglottitis: “cherry red epiglottis”.
- Otitis Media
- Meningitides (only type B)
- Sepsis and Septic arthritis (only in aplesnia)
“cherry red epiglottis
Haemophilus Influenza
Haemophilus Influenza type causes:
TYPE B
sickle cell disease (asplenia) and Haemophilus Influenza
Sepsis and Septic arthritis
Con que se mezcla la vacuna para Hib B?
Vaccine for only the type B capsule is conjugated with diphtheria toxoid and haemophilus type B capsule
Para SINERGIA
Age for Hib B vaccination
6 weeks - 18 mouths
(bound to diphtheria) Dip=Diphtheria
TXT Pertusis
Ceftriaxone
Treatment for close contacts in Pertusis
Rifampin
Stain used in legionella
Silver Stain
Requires agar for Legionella to grow
- Charcoal Yeast
- Cystein
- Iron
Diseases caused by Legionella
- Pontiac Fever
- Legionnaire’s Disease
Pontiac Fever (Legionella)
fever and malaise usually is self-limiting
Common patients with Legionnaire’s Disease
Smokers and elderly men
Infiltrates Legionnaire’s Disease
Atypical pneumonia patchy unilobed infiltrate
Atypical pneumonia patchy unilobed infiltrate,Hyponatremia,headache with confusion , Diarrhea, High fever over 104 F,
Legionnaire’s Disease
Lab test to confirm Legionnaire’s Disease
rapid urine antigen test to confirm
TXT Legionnaire’s Disease
Macrolides and Fluoroquinolones
Oxidase ?? Legionella
Oxidase Positive
Main virulence factor of legionella
Zinc Melloprotease
cytotoxic and inhibits PMN production, inhibits superoxide reduction, deactivates il-1 and CD4 and TNF.
Natural Reservoir Brucella
Cows and Pigs (direct contact)
Veterinarian,butchers,ranchers
Indirect contact with Brucella
Eating chesse or unpasteurized milk
Ondulant fever, chills, and anorexia.
Enlargment of spleen, liver and lymph nodes.
*contact with pigs,cows or eating unpasteurized milk products
Brucelosis
Brucella is a Facultative
Facultative intracellular
Brucella Chronic Infection
Osteomyelitis
TXT Brucella
Tetraciclina/ Doxyciclina + rifampin.
Favorite cell Brucella
Infect macrophages
Urease and H2S positive
Brucella
Require cO2 to grow
Brucella
Gram Neg Coccobacillis
Francisella Telarensis
Haemophilus Influenza
Francisella Telarensis TICK
dermacenter
painful ulcer,causes caseation necrosis - Regional lymphadenopathy
Direct contact with rabbits.
Tularemia
oxidative bursts
Brucella
*Rifampin blocks oxidative burst
not induce oxidative burst
Francisella Telarensis
** Facultative intracellular
Francisella Telarensis Pathogenia
Enters through ulcer and into macrophages in the lymph system to reticuloendothelial organs and causes caseation necrosis
TXT Tularemia
streptomycin
Coxiella burnetii
Causes Q fever
Causes Q fever
Coxiella burnetii
coxiella does not cause a rash
coxiella does not cause a rash
coxiella is an obligate
INTRACELLULAR
coxiella Transmission
- by spores from animal droppings.
* Placental excretions
It gets into humans through aerosol transmission - outbreaks from farm animals to farmers or placental excretions
coxiella
Fever,hepatitis,Hemorrhage on fingers, pneumonia and headache,contact with cows or Ram (cabras).
Q fever
Prevention coxiella
- pasteurization of milk
- No contact with natural reservoirs.
Pastuerella Multocidida is GRAM
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