Bacteria Flashcards
Endure high temperature
Thermoduric
salt tolerant
halophile
acid tolerant
acidophile
cold loving (-40 to -20C)
Psychrophiles
Moderate temp loving (20 to 40C)
Mesophiles
Although they can grow from 20-40 ºC, but the mesophiles would
grow best at ___C that’s why they
can survive in the body.
37
Heat loving (40 to 85C)
Thermophiles
Utilizes inorganic material as source of nutrients
Autotroph
Requires organic material for growth and development
Heterotroph
uses simple inorganic forms
chemolitotrophs
Air (oxygen) requiring
Aerobe
Air (oxygen) poisoned
Anaerobe
Cannot survive in the presence of oxygen
Obligate anaerobe
Can survive with or without oxygen
Facultative anaerobe
Only need a small or minute amount of oxygen to survive
Microaerophilic
Do not need or use oxygen to survive, but they can tolerate or survive in its presence
aerotolerant
Largest bacteria; can grow up to 0.7 mm wide (macroscopic); discovered in Namibia.
Thioimargarita namibiensis
cell shape: These are rounded cells that can divide in different
planes.
cocci
cocci cell shape: Divides on a single plane and remain attached to each
other after division as pairs.
diplococci
cocci cell shape: Divides on a single plane and continues to divide
attached to each other forming a chain.
streptococci
cocci cell shape: Divides on more than three planes and appears as clusters.
Staphylococci
cocci cell shape: Divides on two planes and remain intact as packets of 4.
Tetrad
cocci cell shape: Divides on three planes and appears as packets of 8.
Sarcina
Bacilli divides only on its ___ not on its _____ axis
short axis, longitudinal
Bacilli that divides and separates
Bacillus
Oval-shaped bacilli
Cocobacillus
Bacilli that divides and remain attached
diplobacilli, streptobacilli
bacilli in singly (separate), arranged in palisade
pasilades
_____ are all singles and do not form clusters or chains
spirilla
Spirilla that is an enlarged rod
Fusiform
An example of a helical spirilla
H. pylori
An example of a spirochete spirilla and causative agent of syphilis
treponema pallidum
bacterial cell wall is mainly responsible for maintaining the ___ and __ of the bacteria cell
shape and integrity
_____ are the target of antibiotics
bacterial cell wall
Bacterial cell wall is the target of some antibiotics as they are _____
chemically different with eukaryotic cells
Bacterial cell wall consists of ____ layer which is also called the ____
peptidoglycan, murein
Peptidoglycan is a very important part of the cell wall since it differentiates the different types of microorganisms would help in the identification of bacterial cell
whether it is _______ or _____.
gram positive or gram negative
Generally, the peptidoglycan layer
consists of alternating _____ (NAG) and ________ (NAM), which are attached by peptide bridges.
N-acetyl glucosamine, N-acetyl muramic acid
bacteria that consist of thick peptidoglycan layer and is stained with purple.
Gram positive
Consists of very thin peptidoglycan layer, but it possesses an outer membrane which is mainly made up of lipopolysaccharide.
Gram negative
The outer membrane is easily dissolved in gram staining. Appearing red or pink.
Gram negative
Gram staining process involves?
Decolorizer (alcohol) -> mordant (Gram’s iodine) -> Crystal Violet-Iodine Complex (CVI complex)
_____ (found in the center) is a structure wherein there is no membrane that would bind or enclosed on it.
Nucleoid
____ is a community of different microorganisms. It is a combination of bacteria, fungi, protozoan and maybe some parasites that would protect each
other. This is usually found in canals/sewage.
Biofilm
Glycocalyx can be in the form of ________________ layer.
capsule/slime
____ glycocalyx is well organized while _____ glycocalyx is ruggedly arranged
capsule, slime
The function of capsule is to protect the bacteria form _____, ____, or _____, and it is also a ____ of bacterial cell form the process of phagocytosis.
desiccation, drying, or toxic substances, and it is also the protection
Spores is ___ a method of reproduction because there will be no increase in the number of the bacterial cell.
not
spore to vegetative cell
Germination
Endospores are chemically made up of _____ and ______. It allows the bacterial cell to produce the dormant stage. Because of this endospore formation, the cell becomes high resistant to anything that could affect the cell
dipicolinic acid and calcium
The ________ can survive an environment that would normally kill a bacterial cell in its vegetative form.
endospores
two regions of the endospore
exosporium and cortex
exosporium consist of ___ and __
spore coat and cysteine
cortex consists of ___and ____
peptidoglycan and central core
___ and ___ are genes in the bacterial cell that is activated whenever there is sporulation or endospore formation
spo and ssp
__________ are considered to be the virulence factor of some microorganisms although virulence is not only confined to the ability of the cell to produce toxins since there are other virulence factors.
Bacterial Toxin
Lipid A component of LPS - released from lysed or damaged cell of Gram negative
Endotoxin
Released extracellularly - usually by Gram- positive
Exotoxin
There are bigger number of antibiotics that are used in _______ organisms rather than ________ organism because of the toxic effect
that would happen especially if the target of the bacteria would be the cell wall
gram positive, gram negative
They caused damage to the cell
Cytolytic
consists of cytokines and has massive inflammatory reaction
Super antigen
Bacterial cell multiplication goes therough the process of ______
binary fission
The period wherein the organisms double in number. Around 15 to 20 minutes depending on the type of organism
Generation time
Any material containing essential nutrients for the growth and multiplication of bacteria
Culture media
common bacteria: non-motile, non-spore-forming, catalase positive, carbohydrate fermenter
staphylococcus spp.
common bacteria: white to deep yellow pigment, beta- hemolytic, Gram positive cocci in clusters
staphylococcus spp
causes food poisoning, minor skin infection, TSS
S. Aureus
inhibition of phagocytosis by PMN
CAPSULE
hemolysins, Panton-Valentine leukocidin (MRSA), exfoliative toxin, TSS toxin, enterotoxins are toxins of ____
staphylococcus spp.
lancefield group a
S. pyogenes
“flesh eating bacteria”
S.pyogenes
bacteria that causes acute pharyngitis, scarlet fever, skin infections
s. pyogenes
post sreptococcal, diseases like ______ and ______ may be acquired
rheumatic fever and glomerulonephritis
virulence factor of s. pyogenes
streptolysin O and A
lancefield group b
S. agalactae
bacteria that causes septicaemia, pmeumonia, meningitis
causes s.agalactiae
causes bacterial pneumonia, meningitis, otitis media
s. pneumonia
opportunistic pathogen I n GI and GU tract
viridans streptococcus
a nosocomial infection bacteria
enterococci spp
polysaccharide, pneumolysin, and phosphrylcholine are the virulence factor of _______
s. pneumonia
bacteria spp that is gram positive cocci in chains, catalase negative
streptococcus
bacteria spp that is an opportunistic pathogen I n GI and GU tract
Viridans Streptococcus
Bacteria spp that causes nosocomial infection
Enterococci spp
A spp that is gram positive spore-forming aerobic bacilli in chains
Bacillus spp
Bacillus spp that has a bamboo appearance, spore appear ellipsoidal and are centrally located
B. anthracis
Bacillus that causes Woolsorter’s disease
B. anthracis
B.cereus is an opportunistic pathogen that causes a food-borne illness, ______
progressive endophthalmitis
Bacillus found in feces with no symptoms
B. thuringiensis
B. subtilis is a _____
laboratory contaminant
Actinomycetes and N. braziliensis are G+ that are catalase ____ and have ____ rods
positive, branching
L.monocytogenes and C. diphtheria are catalase ___, ____ fast, ____ former, ____ rods
positive, non-acid, non-spore, non-branching
Enteric organisms are gram _____ bacilli and cocobacilli
negative
Enteric organisms are Mcconkey ____ and oxidase ____
positive, negative
Non lactose fermenters: SSP
Shigella, Salmonella, Proteus
Rapid Lactose Fermenter: EEEK
E.coli, E.aerogenes, E.cloacae, K.pneumoniae
E. coli virulence factors
O Ag, K Ag, Hemolysin
The __ of E.coli causes colonization and infection
O Ag
The ___ of E.coli causes pyelonephritis
K Ag
The ___ is the cytotoxin of the E.coli
Hemolysin
E. coli is the causative agent of ___, ___, and ____
UTI, sepsis, meningitis
E.coli associated diarrheal diseases
EPEC, ETEC, STEC, EIEC, EAEC
E.coli-associated severe watery diarrhea in infants
EPEC
E.coli-associated traveler’s diarrhea
ETEC
E.coli-associated hemorrhagic colitis
STEC
E.coli-associated similar to shigellosis
EIEC
E.coli-associated acute and chronic diarrhea
EAEC
Enteric organism that is non-motile and has mucoid colonies.
Klebsiella spp
Enteric organism that is ampicillin-resistant, has multi-drug resistant plasmids, causes nosocomial infection
Klebsiella
Enteric organism that is motile, positive for ornithine decarboxylase, nosocomial infection
Enterobacter
Component of Enterobacter that makes it resistant to ampicillin, 1st and 2nd gen cephalosporin
ampC
What causes enterobacter to be resistant to 3rd gen cephalosporin
hyperproduce beta-lactamase
Resistant to aminoglycosides, penicillin, and
3rd generation cephalosporins
Serratia
Enteric organism that is has swarming colonies, chocolate cake smell, and a rapid urease producer
Proteus spp
Causative agent of bacteremia, pneumonia, focal lesions, ear infection, and diarrhea
Proteus
Proteus that causes UTI
P.mirabilis
Proteus that causes nosocomial infections
P.vulgaris
Enteric organism that causes nosocomial infection
Morganella spp
Enteric organism that causes UTI
providencia
Enteric organism that causes UTI, sepsis, meningitis, and brain abscess
Citrobacter spp
gene of C. feundii that causes resistance to ampicillin and 1st gen cepha
ampC
non-motile and causes bacillary dysentery
Shigella spp
Virulence factor of Shigella spp
LPS, S.dysenteriae T1
Causative agent of enteritis, systemic infection, and enteric fever
Salmonella
Salmonella that causes enteric fever
S. typhi
S. choleraesuis causes _____
bacteremia with focal lesions
_____ and _____ are the causative agents of enterocolitis
S. typhimurium, and S. Enteritidis
Anaerobic bacteria can either be ___/___ or _____
strict/obligate or aerotolerant
Strict/obligate anaerobes: BFP
Bacteroids, Fusobacterium, Prevotella
Aerotolerant anaerobes: CAB
Clostiridium, Actinomyces, Bafidobacterium
C. perfringes virulence factors
Alpha toxin and Beta toxin
Causative agent of gas gangrene, food poisoning, and necrotizing enteritis
C. perfringens
TeNT is the virulence factor of ____
C. tetani
Causative agent of tetanus
C. tetani
Virulence factor of C. botulinum
BoNT
C. botulinum is the causative agent of ____, _____, ____ botulism
food-born, infant, wound botulism
C. dificile virulence factors
TcdA and TcdB
C. dificile causes
Pseudomembranous colitis
B. fragilis and F. nucleatum virulence factors
Capsule endotoxin and succinic acid
Causative agents of Localized or enclosed abscess, Bacteremia, Aspiration pneumomia, Septic arthritis, Chronic sinusitis, and Decubitus ulcer
B. fragilis and F. nucleatum
spp that are generally aerobic, non-motile, very thin, slightly curved or straight rods
mycobactgerium spp
mycobactgerium spp are non-spore forming except ___
M. marinum