bacteeeeeeeeeeee Flashcards
Microorganisms that are usually isolated from animals
Zoonotics
Campylobacter specie associated with gastritis and diarrhea
C.coli
Microaerophilic and capnophilic gram-negative rods
Campylobacter
they require low oxygen tension, about 5% of oxygen
Microaerophilic
they require high concentration of carbon dioxide,about 5-10 % of carbon dioxide
Capnophilic
Campylobacter optimum temperature for growth
42 C; 36 C- 37 C
Campylobacter motility
cork-screw darting motility
Why do campylobacter have cork-screw darting motility
due to its unipolar or bipolar flagellum
Campylobacter Culture Mediums
Butzler,Skirrows, CAMPY-BAP, CCDA, semisolid motility agasm Campy-CVA
CCDA
Charcoal Cefoperazone Deoxyxholate Agar
CVA
Cephalothin, Vancomycin, Amphotericin B
difference of Skirrow’s medium and Campy-BAP
Skirrows: 5% horse blood
Campy-BAP:5% Sheep blood agar
Components of Skirrow’s Medium
5% Horse Blood , Vancomycin, Polymicin B,Trimethoprim
Campy-BAP and Campy-thio antibiotic components
vancomycin, trimethoprim, cephalotin, polymyxin B, and amphotericin B
Campylobacter Clinical specimens
feces (rectal swabs are also
acceptable for culture) and blood
C. jejuni infection MOT
Ingestion
delays of more than 2 hours require the stool specimen
Cary-Blair transport medium or in
campy thio
campy thio
thioglycollate broth base with:
0.16% agar and vancomycin (10 mg/L)
trimethoprim (5 mg/L)
cephalotin (15 mg/L)
polymyxin B (2500 U/L)
amphotericin B (2 mg/L)
