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Bacte Lab > Bacte Final Quiz > Flashcards

Bacte Final Quiz Flashcards

1
Q

needs are relatively complex, and exceptional media components must be used for growth

A

Fastidious

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2
Q

basic and straightforward needs

A

Nonfastidious

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3
Q

Artificial environment where needs of bacterial organisms are met to proliferate and grow

A

Media

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4
Q

Phases of growth media

A

Broth, Agar, Semi-solid

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5
Q

nutrients are dissolved in water

A

Broth media

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6
Q

bacterial growth is indicated by turbidity

A

Broth Media

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7
Q

Most common solidifying agent

A

Agarose

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8
Q

Agarose melts at what temp?

A

95 C

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9
Q

Agarose solidify after temp. falls below?

A

50 C

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10
Q

Agarose cool around?

A

55-60 C

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11
Q

used to observe and detect the motility of bacteria

A

Semi-solid

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12
Q

provides an indication of the type of organism present based on oxygen requirement

A

Semi-solid

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13
Q

used to enhance growth of a particular bacteria by
providing specific nutrients required
Ex. BYCE (Buffered charcoal-yeast extract agar )- contains L-cysteine for Legionella pneumophila

A

Enrichment media

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14
Q

supports growth if most non-fastidious organism Ex. Tryptic soy agar, nutrient agar

A

Nutritive media

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15
Q

contain inhibitory agents against a specific bacteria, while allowing only a “selected” bacteria to grow

A

Selective media

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16
Q

allows bacteria to exhibit metabolic/culture characteristics that can be distinguished

A

Differential media

17
Q

Broth/agar

A

Brain heart infusion

18
Q

● Purpose: Cultivation of fastidious organisms
● Components: Dextrose, pork brain, and heart
dehydrated infusions

A

Brain Heart Infusion

19
Q

Purpose: Cultivation of fastidious microorganisms such as Haemophilus spp., Brucella spp. and pathogenic Neisseria spp.

A

Chocolate Agar

20
Q

Components: Peptone base, enriched solution of hemoglobin ( hemin “X” factor and Nicotinamide adenine
dinucleotide NAD “V” factor)

A

Chocolate Agar

21
Q

Purpose: Selective isolation of gram-positive cocci

A

Columbia CNA with Blood

22
Q

Components: Peptones sources , 5% defibrinated sheep blood
- Colistin ( C ) - disrupts GN cell membrane
- Nalidixic acid (NA) - blocks DNA replication

A

Columbia CNA with Blood

23
Q

Purpose: Selective (enrichment) liquid medium for enteric pathogens

A

GN Broth

24
Q

Components:
- Mannitol - primary carbon source
- Sodium citrate and Sodium deoxycholate
- bile salts that inhibit GP organisms
- Peptone base broth with Glucose

A

GN Broth

25
Q

Purpose: Differential, selective medium for the isolation and differentiation of Salmonella and Shigella spp. from other gram-negative enteric bacilli
● Components: Peptone-base agar with bile salts, lactose, sucrose, agar salicin, and ferric ammonium citrate; indicators include bromthymol blue and acid fuchsin

A

Hektoen Enteric Agar

26
Q

Purpose: Isolation and differentiation of lactose fermenting and non–lactose- fermenting enteric bacilli

A

Macconkey Agar

27
Q

Components: Peptone base with lactose; gram-positive organisms inhibited by crystal violet and bile salts; neutral red as indicator
* * Lactose Fermenters, Non-lactose fermenters, Slow Fermenters

A

Macconkey Agar

28
Q

Purpose: Selective isolation of aerobic gram-positive cocci and bacilli

A

PhenylEthyl Alcohol Agar

29
Q

Components:
- Nutrient Agar Base
- Phenylethyl Alcohol (inhibits GN)
- 5% Sheep Blood

A

PhenylEthyl Alcohol Agar

30
Q

Purpose: Cultivation of non-fastidious microorganism, determination of hemolytic reactions

A

Sheep Blood Agar

31
Q

Components:
- Trypticase soy agar
- Brucella agar or Beef Heart Infusion
- 5% Sheep Blood

A

Sheep Blood Agar

32
Q

Purpose: Selective for N.gonorrohoeae and N.meningitidis. Supports the growth of Francisella and Brucella spp.

A

MODIFIED THAYER-THAYER MARTIN AGAR

33
Q

Components: Blood agar based enriched with hemoglobin and supplement B
- Colistin = inhibit GN
- Vancomycin = inhibit GP
- Nystatin = inhibit yeast
- Trimethoprim = inhibit Proteus spp.

A

MODIFIED THAYER-THAYER MARTIN AGAR

34
Q

Purpose: Enrichment broth or semi-solid, supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganism

A

THIOGLYCOLLATE BROTH

35
Q

Components:
- Pancreatic digest of casein
- Soy broth
- Glucose

A

THIOGLYCOLLATE BROTH

36
Q

Purpose: Isolation and differentiation of Salmonella and Shigella spp. from other gram negative enteric bacilli
● Components: Yeast extract agar w/ lysine, xylose, lactose & sucrose
- Sodium deoxycholate = inhibits GN
- Phenol Red = pH indicator
- Ferric ammonium citrate = H2s indicator

A

XYLOSE-LYSINE-DEOXYCHOLATE (XLD) AGA

37
Q

Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria.

A

Bile esculin agar (BEA)

Bacte Lab (2 decks)

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