Bacte Final Quiz

Question 1

needs are relatively complex, and exceptional media components must be used for growth

Answer 1

Fastidious

Question 2

basic and straightforward needs

Answer 2

Nonfastidious

Question 3

Artificial environment where needs of bacterial organisms are met to proliferate and grow

Answer 3

Media

Question 4

Phases of growth media

Answer 4

Broth, Agar, Semi-solid

Question 5

nutrients are dissolved in water

Answer 5

Broth media

Question 6

bacterial growth is indicated by turbidity

Answer 6

Broth Media

Question 7

Most common solidifying agent

Answer 7

Agarose

Question 8

Agarose melts at what temp?

Answer 8

95 C

Question 9

Agarose solidify after temp. falls below?

Answer 9

50 C

Question 10

Agarose cool around?

Answer 10

55-60 C

Question 11

used to observe and detect the motility of bacteria

Answer 11

Semi-solid

Question 12

provides an indication of the type of organism present based on oxygen requirement

Answer 12

Semi-solid

Question 13

used to enhance growth of a particular bacteria by
providing specific nutrients required
Ex. BYCE (Buffered charcoal-yeast extract agar )- contains L-cysteine for Legionella pneumophila

Answer 13

Enrichment media

Question 14

supports growth if most non-fastidious organism Ex. Tryptic soy agar, nutrient agar

Answer 14

Nutritive media

Question 15

contain inhibitory agents against a specific bacteria, while allowing only a “selected” bacteria to grow

Answer 15

Selective media

Question 16

allows bacteria to exhibit metabolic/culture characteristics that can be distinguished

Answer 16

Differential media

Question 17

Broth/agar

Answer 17

Brain heart infusion

Question 18

● Purpose: Cultivation of fastidious organisms
● Components: Dextrose, pork brain, and heart
dehydrated infusions

Answer 18

Brain Heart Infusion

Question 19

Purpose: Cultivation of fastidious microorganisms such as Haemophilus spp., Brucella spp. and pathogenic Neisseria spp.

Answer 19

Chocolate Agar

Question 20

Components: Peptone base, enriched solution of hemoglobin ( hemin “X” factor and Nicotinamide adenine
dinucleotide NAD “V” factor)

Answer 20

Chocolate Agar

Question 21

Purpose: Selective isolation of gram-positive cocci

Answer 21

Columbia CNA with Blood

Question 22

Components: Peptones sources , 5% defibrinated sheep blood
- Colistin ( C ) - disrupts GN cell membrane
- Nalidixic acid (NA) - blocks DNA replication

Answer 22

Columbia CNA with Blood

Question 23

Purpose: Selective (enrichment) liquid medium for enteric pathogens

Answer 23

GN Broth

Question 24

Components:
- Mannitol - primary carbon source
- Sodium citrate and Sodium deoxycholate
- bile salts that inhibit GP organisms
- Peptone base broth with Glucose

Answer 24

GN Broth

Question 25

Purpose: Differential, selective medium for the isolation and differentiation of Salmonella and Shigella spp. from other gram-negative enteric bacilli ● Components: Peptone-base agar with bile salts, lactose, sucrose, agar salicin, and ferric ammonium citrate; indicators include bromthymol blue and acid fuchsin

Answer 25

Hektoen Enteric Agar

Question 26

Purpose: Isolation and differentiation of lactose fermenting and non–lactose- fermenting enteric bacilli

Answer 26

Macconkey Agar

Question 27

Components: Peptone base with lactose; gram-positive organisms inhibited by crystal violet and bile salts; neutral red as indicator * * Lactose Fermenters, Non-lactose fermenters, Slow Fermenters

Answer 27

Macconkey Agar

Question 28

Purpose: Selective isolation of aerobic gram-positive cocci and bacilli

Answer 28

PhenylEthyl Alcohol Agar

Question 29

Components: - Nutrient Agar Base - Phenylethyl Alcohol (inhibits GN) - 5% Sheep Blood

Answer 29

PhenylEthyl Alcohol Agar

Question 30

Purpose: Cultivation of non-fastidious microorganism, determination of hemolytic reactions

Answer 30

Sheep Blood Agar

Question 31

Components: - Trypticase soy agar - Brucella agar or Beef Heart Infusion - 5% Sheep Blood

Answer 31

Sheep Blood Agar

Question 32

Purpose: Selective for N.gonorrohoeae and N.meningitidis. Supports the growth of Francisella and Brucella spp.

Answer 32

MODIFIED THAYER-THAYER MARTIN AGAR

Question 33

Components: Blood agar based enriched with hemoglobin and supplement B - Colistin = inhibit GN - Vancomycin = inhibit GP - Nystatin = inhibit yeast - Trimethoprim = inhibit Proteus spp.

Answer 33

MODIFIED THAYER-THAYER MARTIN AGAR

Question 34

Purpose: Enrichment broth or semi-solid, supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganism

Answer 34

THIOGLYCOLLATE BROTH

Question 35

Components: - Pancreatic digest of casein - Soy broth - Glucose

Answer 35

THIOGLYCOLLATE BROTH

Question 36

Purpose: Isolation and differentiation of Salmonella and Shigella spp. from other gram negative enteric bacilli ● Components: Yeast extract agar w/ lysine, xylose, lactose & sucrose - Sodium deoxycholate = inhibits GN - Phenol Red = pH indicator - Ferric ammonium citrate = H2s indicator

Answer 36

XYLOSE-LYSINE-DEOXYCHOLATE (XLD) AGA

Question 37

Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria.

Answer 37

Bile esculin agar (BEA)