Exam

Question 1

Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria.

Answer 1

Bile esculin agar (BEA)

Question 2

Differential isolation and presumptive identification of group D streptococci and enterococci

Answer 2

BEA

Question 3

Contains azide to inhibit gram-negative bacteria, vancomycin to select for resistant gram-positive bacteria, and bile esculin to differentiate entero- cocci from other vancomycin-resistant bacteria that may grow

Answer 3

Bile esculin azide agar with vancomycin

Question 4

Selective and differential for cultivation of vancomycin-resistant enterococci from clinical and surveillance
specimens

Answer 4

Bile esculin azide agar with vancomycin

Question 5

Trypticase soy agar, Brucella agar, or beef heart infusion with 5% sheep blood

Answer 5

Blood Agar

Question 6

Cultivation of nonfastidious microorgan- isms, determination of hemolytic reactions

Answer 6

BA

Question 7

Potato-glycerol–based medium enriched with 15%- 20% defibrinated blood; contaminants inhibited by methicillin (final concentration of 2.5 μm/mL)

Answer 7

Bordet-Gengou agar

Question 8

Isolation of Bordetella pertussis and Bordetella parapertussis

Answer 8

Bordet-Gengou agar

Question 9

Yeast extract, agar, charcoal, and salts supplemented with L-cysteine HCl, ferric pyrophosphate, ACES buffer, and alpha-ketoglutarate

Answer 9

Buffered charcoal–yeast extract agar (BCYE)

Question 10

Enrichment for Legionella spp. Supports the growth of Francisella and
Nocardia spp.

Answer 10

BCYE

Question 11

BCYE supplemented with polymyxin B, vancomycin, and ansamycin to inhibit gram-negative bacteria, gram-positive bacteria, and yeast, respectively

Answer 11

Enrichment for Legionella spp. Supports the growth of Francisella and
Nocardia spp.

Question 12

Enrichment and selection for Legionella spp.

Answer 12

Buffered charcoal–yeast extract (BCYE) agar with antibiotics

Question 13

Bile salts, gentamicin, ticarcillin, polymyxin B, peptone, yeast extract

For recovery of B. cepacia from cystic fibrosis patients

Answer 13

Burkholderia cepacia
selective agar

Question 14

Contains vancomycin (10 mg/L), trimethoprim
(5 mg/L), polymyxin B (2500 U/L), amphotericin B (2 mg/L), and cephalothin (15 mg/L) in a Brucella agar base with sheep blood

Selective for Campylobacter spp.

Answer 14

Campy-blood agar

Question 15

Thioglycollate broth supplemented with increased agar concentration and antibiotics

Selective holding medium for recovery of Campylobacter spp.
Incubated at 4°C for cold-enrichment

Answer 15

Campylobacter thioglycollate broth

Question 16

Tryptic soy broth with phenyl-ethyl alcohol, 5% sheep blood, and added nutrients

Improved growth of obligate, slow- growing anaerobes

Answer 16

CDC* anaerobe 5% sheep blood agar

Question 17

Blood-supplemented enrichment medium containing cefoperazone, vancomycin, and amphotericin to inhibit growth of most gram-negative bacteria, gram-positive bacteria, and yeast, respectively

Selective medium for isolation of Campylobacter spp.

Answer 17

Cefoperazone, vancomy- cin, amphotericin (CVA) medium

Question 18

Peptone base with yeast extract, mannitol, and bile salts; supplemented with cefsulodin, irgasan, and novobiocin; neutral red and crystal violet indicators

Selective for Yersinia spp.; may be useful for isolation of Aeromonas spp

Answer 18

Cefsulodin-irgasan- novobiocin (CIN) agar

Question 19

Organism-specific nutrient base, selective supple- ments, and chromogenic substrate

Designed to optimize growth and differ- entiate a specific type of organism; routinely used in the identification of yeasts, methicillin-resistant Staphylo- coccus aureus (MRSA), and a variety of other organisms

Answer 19

Chromogenic media