BACTE CULTURE AND CULTURE MEDIA

Question 1

General Steps in Preparation of Culture Medium: TUBE METHOD

Answer 1

1. Weighing
2. Dissolving
3. Titration
4. Distribution
5. Sterilization

Question 2

General Steps in Preparation of Culture Medium: PLATED MEDIUM

Answer 2

1. Weighing
2. Dissolving
3. Titration
4. Sterilization
5. Distribution

Question 3

Sterile body fluids, pus, urine and sputum

Answer 3

inoculated directly into the selected media

Question 4

Specimens received on swabs

Answer 4

can be inoculated directly into the culture media

Question 5

Specimens that require direct or “bedside” inoculations:

Answer 5

Blood, Genital specimens, Corneal Scrapings,
Sterile Fluids like Synovial and Peritoneal Fluids
and Nasopharyngeal Swabs for isolation of
Bordetella pertusis

Question 6

most common manner of inoculating

Answer 6

streaking