Bacillus

Question 1

Comet tail, Medusa head, ground glass colonies in BAP

Answer 1

Bacillus anthracis

Question 2

Capable of spore-forming within mother cell or endospores

Answer 2

Bacillus anthracis

Question 3

Most common form worldwide, characterized by necrotic skin lesions called black eschars

Answer 3

Cutaneous anthrax

Question 4

Spread by inhalation of spores from sheep’s wool

Answer 4

Pulmonary anthrax “wool-sorter’s disease”

Question 5

Motile, penicillin resistant, beta-hemolytic

Answer 5

B. cereus

Question 6

Toxins produced by B. anthracis

Answer 6

Lethal toxin, edema toxin

Question 7

Crystal-forming

Answer 7

B. thuringiensis

Question 8

Cold-tolerant

Answer 8

B. weihenstephanensis

Question 9

Heat-tolerant

Answer 9

B. cytotoxicus

Question 10

Probiotic

Answer 10

B. toyonensis

Question 11

food poisoning by ingestion of variety of foods
(ESP. RICE DISHES)

Answer 11

B. cereus

Question 12

Toxins produced by B. cereus

Answer 12

Hemolysin BL (HBL)
Nonhemolytic enterotoxin (Nhe) Cytotoxin K (CytK; Hemolysin IV) Proteolysis (Heat stable)

Question 13

Occupational exposure to insecticide and pesticides containing organism has resulted in ID of organism in feces without presence of GI symptions

Answer 13

B. thuringiensis

Question 14

Incubation period of cutaneous anthrax

Answer 14

2-6 days

Question 15

Pretreatment used before plating to remove contaminating organisms, and only the spore-forming bacilli survive

Answer 15

Heat or alcohol shock

Question 16

For removal of contaminating organisms and isolation of Bacillus spp

Answer 16

Phenylethyl alcohol agar (PEA)

Question 17

Induce B. anthracis capsule formation. Requires incubation in CO2

Answer 17

Bicarbonate agar

Question 18

Parasoporal crystals

Answer 18

B. thuringiensis

Question 19

Incubation requirement for bacillus

Answer 19

Growth within 24 hrs after incubation @ 35C in ambient air, or in 5% CO2

Question 20

Enzyme that bacilli produce

Answer 20

Lecithinase