B7 - DNA fingerprinting Flashcards
1
Q
What is special about the 2 strands of the DNA molecule?
A
They are complimentary (fit together)
2
Q
Where would you get DNA for testing? Which cells?
A
Blood test, only the white blood cells have DNA.
3
Q
What happens to the DNA when doing DNA testing?
A
- Isolate DNA from cells
- Cut DNA up using enzymes.
4
Q
How are the two strands of DNA separated?
A
THe DNA is heated, the protein is denatured, so the strands stay apart.
5
Q
What is a gene probe?
A
A piece of DNA that “fits” onto the DNA that is being tested for.
6
Q
What does the gene probe do?
A
Attaches to the base sequence that is being looked for.
7
Q
How are the DNA fragments separated out?
A
Gel Electrophoresis - a bit like chromatography,
8
Q
How is the result of the DNA test read?
A
UV light reacts with the gene probe
