B5-BIOLOGICAL AGENTS Flashcards
A large livestock farm employs a number of people who are involved in caring for animals, cleaning out animal enclosures and disposing of waste materials.
The farm manager has identified that during such work employees may
be exposed to zoonoses.
(a) Give the meaning of the term ‘zoonose’. (2)
A ‘zoonose’ may be defined as an animal disease or infection that may be transmitted
to humans. Whilst the disease or infection will cause ill-health to humans, it may not
necessarily cause ill-health in animals.
(b) Cryptosporidiosis and Leptospirosis are two commonly occurring zoonoses.
Outline how and when the farm employees are likely to be exposed to EACH of these. (6)
farm employees are likely to be
exposed to cryptosporidiosis when in contact with cattle or sheep.
The exposure may occur via ingestion following hand to mouth transmission, through contact with surfaces or footwear contaminated with faeces while clearing out enclosures, and when there is a poor standard of personal hygiene such as a failure to carry out regular hand washing.
As for leptospirosis, exposure may take place after contact with cattle or rat urine via broken skin such as cuts or grazes, or via the mucous membranes in the eye, nose or mouth when cattle urine has splashed into an
individual’s face.
(c) Outline a range of practical control measures that should be
used to minimise the risks associated with exposure to zoonoses. (10)
the necessity for employees to practise a high standard of personal hygiene;
the provision of rest areas away from the animals provided with clean drinking water and separate hand washing facilities including hot water, soap and paper towels or a hand
drier so that employees may wash their hands and arms before eating and drinking;
avoiding the use of tools or equipment likely to damage the skin and if damage should occur, covering the resultant cuts or grazes with waterproof dressings;
maintaining good standards of hygiene in livestock areas by regular cleaning and
disinfecting and the removal of faeces;
wearing suitable personal protective
equipment such as gloves and overalls, eye and face protection to avoid urine
splashes and respiratory protective equipment to avoid aerosol inhalation;
leaving work wear and personal protective equipment at the workplace for cleaning;
controlling the disease in the animals for example by vaccination;
and
ensuring that all employees are made fully aware of the risks of exposure to the disease when handling cattle and the precautions that must be observed.
(d) Identify TWO other zoonoses that employees working on the farm could be exposed to. (2)
a list comprising bovine tuberculosis, salmonella, streptococcus suis, orf, psittacosis, Q fever and ringworm.
(a) The risk of contracting Weil’s Disease (Leptospirosis) is a concern to windsurfers at a local water sports centre.
(i) Outline the ill-health effects associated with this disease (2)
flu-like symptoms - headaches / myalgia of lower back and legs / fever / nausea / sickness / vomiting + diarrhoea / skin rash / conjunctival haemorrhage / liver failure / jaundice / meningitis / death
(a) The risk of contracting Weil’s Disease (Leptospirosis) is a concern to windsurfers at a local water sports centre.
(ii) Outline why windsurfers might be at risk (2)
windsurfers may be at risk because:
- presence of rodents around water courses
- rat urine carries bacterial agent leptospira ichterohaemorrhagia
- bacteria remains viable in water • windsurfers spend prolonged periods of time in water
- bacteria can enter skin through cuts / abrasions / mucous membranes - nose / eyes
- infection also possible via ingestion - swallowing water
(b) The risk of contracting Weil’s Disease (Leptospirosis) is a concern to windsurfers at a local water sports centre.
Outline the practical steps instructors can take to minimise the risks to themselves (6)
risks to instructors can be minimised by:
- minimising water contact where practicable - conduct tuition from boat
- hygiene - washing hands / showers after water contact and before eating etc
- covering cuts with waterproof dressings
- disinfecting equipment after use
- wearing full dry (wet?) suit and footwear
- ensure appropriate training and information provided on health risks and control measures
- display warning signs
- carry medical information card for GP
- report rodent sightings etc and institute pest control
- minimise foodstuffs / debris around area
a) Summarise the specific criteria that should be applied when provisionally classifying a biological agent in accordance with the Control of Substances Hazardous to Health Regulations 2002. (4)
Biological agents are allocated to Hazard Groups 1 (least hazardous - not covered by COSHH) to 4 (most hazardous) in accordance to the WHO classification criteria - these being:
- how easily the agent can infect an individual (individual risk)
- how severe the resulting disease / adverse health effect is (severity)
- how easily the disease is transmitted to others (community risk)
- how easily the disease is treated (availability of treatment / prophylaxis)
(b) Outline the design requirements for a research laboratory that undertakes activities involving a Group 4 biological agent. (6)
The design requirements for a lab dealing with HG 4 pathogens include:
- restricted entry to nominated personnel only via pass / key
- posting of biohazard signs
- lab at negative pressure to outside environment (leaks inward); airlock entry system
- provision of appropriate secure storage cabinets
- provision of microbiological safety cabinets / LEV
- changing facilities / segregated areas
- surfaces to be sealed and impervious to assist cleaning and disinfection
- surfaces to be resistant to acids / alkalis etc
- safe disposal facilities for contaminated waste - segregated containment
- provision of hygiene facilities - showers, sinks, anti-bacterial soap, hot water
- equipment / machinery to be fixed - not removable
- viewing window to lab from exterior
A large livestock farm employs a number of people who are involved in caring for animals, cleaning out animal enclosures and disposing of waste materials.
The farm manager has identified that such work employees may be exposed to zoonoses.
(a) Give the meaning of the term “zoonose”. (2)
Disease / infection that can be passed from animal to human (may not cause illness in animal but does in human
A large livestock farm employs a number of people who are involved in caring for animals, cleaning out animal enclosures and disposing of waste materials.
(b) Cryptosporidiosis and Leptospirosis are two commonly occurring zoonoses. Outline how and when the farm employees are likely to be exposed to each of these. (6)
Leptospirosis: via open cuts / wounds following contact with / splash of rat or cattle urine containing leptospira bacteria;
sometimes through splash to mucous membrane - nose/ eye.
Cryptosporidium: via ingestion following contact with contaminated sheep / cattle dung / bedding etc
(poor hygiene / no washing / eating / fingers in mouth etc) or via ingestion of contaminated water.
A large livestock farm employs a number of people who are involved in caring for animals, cleaning out animal enclosures and disposing of waste materials.
(c) Outline a range of practical control measures that should be used to minimise the risks associated
with exposure to zoonoses. (10)
the necessity for employees to practise a high standard of personal hygiene;
the provision of rest areas away from the animals provided with clean drinking water and separate hand washing facilities including hot water, soap and paper towels or a hand
drier so that employees may wash their hands and arms before eating and drinking;
avoiding the use of tools or equipment likely to damage the skin and if damage should occur, covering the resultant cuts or grazes with waterproof dressings;
maintaining good standards of hygiene in livestock areas by regular cleaning and
disinfecting and the removal of faeces;
wearing suitable personal protective
equipment such as gloves and overalls, eye and face protection to avoid urine
splashes and respiratory protective equipment to avoid aerosol inhalation;
leaving work wear and personal protective equipment at the workplace for cleaning;
controlling the disease in the animals for example by vaccination;
and
ensuring that all employees are made fully aware of the risks of exposure to the disease when handling cattle and the precautions that must be observed.
A large livestock farm employs a number of people who are involved in caring for animals, cleaning out animal enclosures and disposing of waste materials.
(d) Identify two other zoonoses that employees working on a farm could be exposed to. (2)
Bovine TB; Orf; Q fever; psittacosis; ringworm
Street cleaning operatives working for a local authority can be at risk of exposure to the Hepatitis B virus.
Outline a range of practical control measures that could be used to minimise the risk of exposure to hepatitis B. (10)
the use of litter picking sticks to minimise the risk of puncture wounds;
the prohibition of eating and drinking during normal periods of work;
the use of personal protective equipment
such as goggles, gloves and footwear;
covering up wounds with waterproof dressings;
the safe disposal of sharps in a suitable container and frequent hand washing.
In addition to these practical controls, candidates could have mentioned the need to
provide information and training to the employees on the nature of the risk and the
precautions to be taken together with the introduction of procedures for the collection
of contaminated waste such as body fluids, blood and contaminated clothing;
for cleaning up body fluids and blood and for the action to be taken following a needle
stick injury such as the encouragement of bleeding and rinsing the wound under warm
water before covering it with a dressing.
A company that operates hotels and health spas recognises the risks associated with the legionella bacteria.
(a) Identify specific locations where there may be growth of or potential exposure to legionella for employees and guests. (5)
Potential locations of growth or potential exposure to legionella include:
hot and cold water storage and transfer systems where the water temp is between 25-40C and contaminated water droplets / aerosols are generated - eg taps, shower heads, saunas, spa baths, hot-tubs, whirlpools, “jacuzzis”, steam rooms, laundry rooms, fountains, ornamental water cascades, fire sprinkler systems, garden
sprinklers, wet or condenser air conditioning systems , cooling towers etc and any areas where there is stagnant water / deadlegs.
Spa bath temperatures (30-40C), presence of dead skin cells / hair etc (nutrient supply) and agitation of water (air jets etc) create ideal conditions for legionella proliferation and increase the risk of inhalation.
(b) Outline the control measures that this company should implement to minimise exposure to
legionella bacteria. (15)
Requirements for managing the risk from legionella are set out in the ACOP Control of legionella bacteria in water systems (L8).
These include:
appoint a competent / responsible person to manage the water system;
replace wet air-conditioning systems with dry if RP;
design the system to minimise
deadlegs and stagnant water;
avoid use of rubber / wood etc (materials that harbour bacteria and are difficult to clean effectively);
regular water treatment / disinfection / use of biocides, chlorination etc of spa pools and other systems;
regularly check / inspect / test / sample water to monitor biocide levels, pH, flow rates, temperature and bacterial levels (dip slides sent to lab for analysis);
regular cleaning & disinfection of storage tanks, heaters and calorifiers;
remove slime, mould, biofilms;
inspection of storage tanks for signs of contamination - algae / debris etc;
keep hot water temp above 60C (but risk
of scalds) and cold water temp below 20C;
train employees in hazards / risks / cleaning procedures / dosing procedures / hazard recognition / keeping records;
cleaning taps / sprinklers / shower heads - removing scale / slime;
running infrequently used taps / showers for several minutes to flush system; ;
running taps / showers for several minutes immediately before occupation of a room; use of drift eliminators on cooling towers.
According to current veterinary and medical opinion the bacterium E.Coli 0157 should be assumed to be carried by all ruminants (cattle, sheep, goats, deer).
In addition, this bacterium can exist for long
periods of time outside of the animal (in soil and faeces).
(a) Identify ways in which children making an educational visit to an open farm might be exposed to E.Coli 0157 bacteria. (4)
Transmission is via ingestion (NOT via cuts / abrasions) so generally arising from hand-mouth contact.
Therefore exposure could occur via touching, stroking, petting, kissing animals - or touching
fences, enclosures, soil, animal bedding, contaminated footwear etc. - followed by eating / drinking / biting nails / sucking fingers without thoroughly washing hands.
According to current veterinary and medical opinion the bacterium E.Coli 0157 should be assumed to be carried by all ruminants (cattle, sheep, goats, deer).
In addition, this bacterium can exist for long
periods of time outside of the animal (in soil and faeces).
(b) Explain a range of practical control measures that should be put in place in order to reduce this risk of exposure. (6)
Practical controls include:
briefing / information to schools / parents / children prior to visit; induction
session on arrival (+ brief details);
restricted access to animals / segregation of animals - barriers / fencing;
hygiene arrangements - regular cleaning of animal pens;
hygiene facilities located before entry to segregated eating areas;
washing hands before eating etc; signs / warnings;
supervision of children.
Comment: Suggestions for full PPE / RPE, perspex screens between animals and children and substitution with stuffed or robotic animals were not considered appropriate!