B4 - Biological molecules Flashcards
types of digestive enzymes
protease enzymes - pepsin (stomach) and trypsin (duodenum)
amylase (saliva and pancreatic)
lipase
describe enzymes
proteins that are involved in all metabolic reactions, where they function as biological catalysts
enzyme activity parts (5)
enzyme
substrate
enzyme-substrate complex
active site
product
describe the test for starch (3)
add iodine
positive test is colour turns blue-black
negative test is colour stays yellow-brown
describe the test for reducing sugars (4)
add Benedict’s solution
heat solution
positive test is colour turns green-yellow-orange-brick red
negative test is colour stays blue
describe the test for protein (5)
called the biuret test
add potassium hydroxide to make solution alkaline
add copper sulfate
positive test is colour turns purple
negative test is colour stays blue
describe the test for lipids
called the ethanol emulsion test
shake substance with ethanol
pour solution into water
positive test is solution precipitates and shows up at the top as a milky emulsion
negative test is no cloudy emulsion formed at the top