B3 Digestion Flashcards
Test for starch.
Test for starch:
1 Place a small amount of food on the spotting tile.
2 Add a few drops of iodine solution to the food on the
spotting tile.
3 Yellow–red iodine solution turns blue–black if starch is
present.
4 Record your result in the results table.
5 Repeat steps 1–4 for other types of food.
Test for sugar.
Test for sugar:
1 Place a small amount of food in a test tube.
2 Add enough Benedict’s solution to cover the food.
3 Place the test tube in a warm water bath for 10 minutes.
4 Blue Benedict’s solution turns brick red on heating if a
sugar such as glucose is present.
5 Record your result in the results table.
6 Repeat steps 1–5 for other types of food.
Test for lipids.
Test for lipids (fat):
1 Place a small amount of food into a test tube.
2 Add a few drops of ethanol to the test tube.
3 Shake the test tube and leave for 1 minute.
4 Pour the solution into a test tube of water.
5 Ethanol added to a solution gives a cloudy white layer if
a lipid is present.
6 Record your result in the results table.
7 Repeat steps 1–6 for other types of food.
Test for protein
Test for protein:
1 Place a small amount of food in a test tube.
2 Add 1 cm
3
of Biuret reagent. Alternatively add 1 cm
sodium hydroxide solution and then add a few drops of
copper sulfate solution.
3 Blue Biuret reagent turns purple if a protein is present.
4 Record your result in the results table.
5 Repeat steps 1–4 for other types of food.
active site
the site on an enzyme where the reactants bind
amino acids
molecules made up of carbon, hydrogen, oxygen, and nitrogen that are the building blocks of proteins
amylase
enzyme that speeds up the digestion of starch into sugars
bile
neutralises stomach acid to give a high pH for the enzymes from the pancreas and small intestine to work well. It is
not an enzyme
carbohydrases
enzymes that speed up the breakdown of carbohydrates into simple sugars
carbohydrates
molecules that contain only carbon, hydrogen, and oxygen. They provide the energy for the metabolism and are
found in foods such as rice, potatoes, and bread
catalyst
a substance that speeds up the rate of another reaction but is not used up or changed itself
denatured
the breakdown of the molecular structure of a protein so it no longer functions
differentiate
the process where cells become specialised for a particular function
digestive system
organ system where food is digested and absorbed
enzymes
biological catalysts, usually proteins