B1.1 Flashcards

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1
Q

What is the difference between prokaryotic and eukaryotic cells?

A

-Eu have nuclei which stores genetic material,pro have no nuclei, the genetic material floats in cytoplasm
-Eu are bigger
Prokaryotes are complex.

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2
Q

What are the different main structures in an animal (eu) cell

A

Cytoplasm,nucleus,mitochondria,cell membrane

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3
Q

What extra structures do plant cells have compared to animals?

A

Vacuole, cell wall, chloroplasts

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4
Q

How many micrometers (um) in a meter?

A

1000000(1 x 10^6)

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5
Q

What is one way you can observe bacteria growth?

A

On an agar plate

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6
Q

Why dont potato cells have chloroplasts?

A

They grow underground
So they dont receive light
So they dont photosynthesise as light is needed for it
So they dont need chloroplasts as photosynthesis never happens

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7
Q

How do you adjust the magnification and stage position in microscopy

A

Magnification - low to high
Stage - moving away from lens

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8
Q

Whats the main structure that gives a bacterial cell(pro) its genetic info

A

A single long, somewhat circular strand of DNA called the (bacterial) chromosome

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9
Q

Describe a plasmid

A

A circle of DNA, storing extra genes.They are helpful for times of stress, such as resistance genes

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10
Q

What is a flagellum?

A

Tail like structures which help for movement through liquids

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11
Q

What are pili?

A

Hair like structures that enable the cell to attach to substances, can also transfer genetic material

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12
Q

What is a slime capsule?

A

A layer outside the cell wall preventing the bacteria from drying out,also helps in sticking to surfaces

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13
Q

Why would you stain a cell?

A

Many cells are colourless or transparent,so stains are used to give them colour and make them easier to observe

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14
Q

What stain for what specimen?

a - methylene blue
b - iodine
c - crystal violet

A

a - animal cells
b - plant cells
c - bacterial cells

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15
Q

What is the smallest difference between 2 points seen as separate entities?

A

Resolution

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16
Q

In light microscopy,why do specimen need to be thin

A

For images to be produced,a light shines through the specimen,so it needs to be thin

17
Q

Advantages and disadvantages of an electron microscope

A

Positive - high resolution, false colour can be added, high magnification
Negative - Expensive, large, needs training to prepare samples, cannot examine live specimen

18
Q

TEM vs SEM

A

Transmission/Scanning are both electron microscopes, but scanning produces in 3D instead of 2D. TEM sends electrons through the specimen, SEM sends them across the surface

19
Q

How do you apply a stain on a cell?

A
  1. Place the cells on a glass slide
  2. Add on drop of stain
  3. Place a coverslip on top
  4. Tap the coverslip gently with a pencil to remove air bubbles.
20
Q

What are the seven life processes every cell can carry out?

A

M - movement
R - reproduction
S - sensitivity
G - growth
R - respiration
E - excretion
N - nutrition