B1 Required Practical

Question 1

Step 1

Answer 1

Use a dropping pipette to put a drop of water onto a glass slide.

Question 2

Step 2

Answer 2

Separate one of the thin layers of the onion.

Question 3

Step 3

Answer 3

Using forceps peel off a thin layer of epidermal tissue from the inner surface.

Question 4

Step 4

Answer 4

Place this thin layer on to the drop of water that you have placed on the microscope slide.

Question 5

Step 5

Answer 5

Make sure that the layer of onion cells is flat
on the slide.

Question 6

Step 6

Answer 6

Put 2-3 drops of iodine solution onto the onion tissue.

Question 7

Step 7

Answer 7

Carefully lower a coverslip onto the slide. Do this by:

Question 8

Step 8

Answer 8

8. Use a piece of blotting paper to soak any excess liquid on the slide.

Question 9

Step 9

Answer 9

Put the prepared slide on the microscope Stage