B1: Cells Flashcards
What are features of a light microscope?
Cheaper to make
Allow you to see the outline of cells
Uses light to form images
Living samples can be viewed
Low magnification
Low resolution
What are features of electron microscopes?
Can visualise finer details including organelles
Higher magnification
Higher resolution
Samples cannot be living
Use beam of electrons to form images
Expensive
What is the equation for magnification?
Magnification = image size / object (cell) size
How big are cells?
A few micrometers long
1 micrometer = 1x10^-6
How to you convert micrometers to mm?
Divide by 1000
Where is DNA found in eukaryotic cells?
In the nucleus
What are 2 examples of eukaryotic cells?
Animal cells
Plant cells
What are organelles?
Sub-cellular structures
Where is DNA in prokaryotic cells found?
A single loop of DNA
As plasmids (small loops of DNA)
What is an example of a prokaryotic cell?
Bacteria
What is the cell membrane?
Semi-permeable
Controls movement of substances in and out of a cell
What is the cell wall made of and what is it’s function?
Cellulose
Strengthens the cell
What takes place within the mitochondria?
Respiration to release energy
What is the function of ribosomes?
Site of protein synthesis
What are chloroplasts?
Made of chlorophyll that absorbs light energy for photosynthesis
What is stored within the permanent vacuole?
Cell sap
How do bacteria reproduce?
By binary fission
They divide every 10 minutes
How do we make cultures with aseptic technique?
-Lift lid of petri dish towards flame (to sterilise air by moving microbes away/killing them)
-Put drop of culture on agar/spread evenly
-Put drops/discs of antibiotics on culture if needed
-Few bits of tape (to allow aerobic respiration), incubate at 25 degrees celsius
-Measure size of cultures/areas with no bacteria with pi x r^2 or pi x d / 4
How many pairs of chromosomes do diploid cells have?
23
How many chromosomes are in gametes?
23
What is a haploid cell?
A cell with 23 singular chromosomes