B1 - Cell Biology Flashcards
Describe the differences between eukaryotes and prokaryotes
eukaryotes: contain their genetic material enclosed in a nucleus
prokaryotes: no nucleus to enclose genetic material, much smaller than eukaryotes.
(Describe function of) nucleus
(What) contains genetic material that controls the activities of the cell
cytoplasm
jelly like substance where most of chemical reactions take place
cell membrane
holds the cell together and controls what enters and leaves the cell
mitochondria
site of aerobic respiration
ribosomes
Site of protein synthesis
cell wall
structure made of cellulose which supports and strengthens the cell
Permanent vacuole
contains cell Sap which gives the cell its shape
Chloroplasts
contain green pigment called chlorophyll which absorbs light energy for photosynthesis
what do plant cells contain that animal cells don’t
chloroplast
cell wall
permanent vacuole
do bacteria cells have a nucleus, if not what do they have instead
No, Dna loops float freely in cytoplasm
Describe how to set up and use a microscope
- Place your prepared slide onto the stage and use the clips to hold it in place
- Select the lowest power objective lens
- Use the coarse focussing knob to move the stage up, just below the objective lens
- Look down the eyepiece and slowly use the coarse focussing knob to move the slide downwards until the image is roughly in focus
- Use the fine focussing knob to adjust the focus until you get a clear image
- If you need to see the slide with greater magnification, swap to higher powered objective lens and repeat the steps until focus.
Differences between light and electron microscope
Electrons: higher magnification and resolution, allows to see more detail of internal structures
Light: let us see individual cells and large sub cellular structures, uses light.
Formula for magnification
Magnification = image / real
why do we use a single layer of epidermal tissue
to see through the cell clearly and so that there is contrast under the microscope