Autopsy and Laboratory Diagnosis Flashcards

1
Q

Personal protective equipment to ensure biosafety when doing a necropsy

A
  • Gloves
  • Coveralls
  • Boots
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2
Q

Why do a postmortem?

A
  • Confirm/reject clinical diagnosis
  • Dealing with unknown disease = identify what disease processes are occuring to explain cause of death or clinical findings, collect tisues to conduct tests which will establish a cause (information may be needed to prevent or treat disease in other animals)
  • Testing for agents without documenting pathological process is of limited use
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3
Q

When are you going to do a postmortem?

A

-As soon as possible

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4
Q

When are you going to examine other animals?

A

-Before doing a necropsy on the dead animals

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5
Q

Which animals will you do a postmortem on?

A

Dead ones

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6
Q

Where will you do the postmortem?

A
  • Concrete
  • Dirt area in sun
  • Straw bed
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7
Q

What will you do with the carcasses?

A
  • Burial
  • Renderer
  • Transport to a disposal site
  • Incineration
  • Composting
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8
Q

Areas to AVOID while doing a postmortem

A
  • Areas accessible to animals
  • Areas which may contain food
  • High traffic areas
  • Areas difficult to disinfect
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9
Q

GREAT postmortem sites

A
  • Concrete
  • Dirt area in sun
  • Straw bed
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10
Q

Disposal options

A
  • Burial
  • Renderer
  • Transport to a disposal site
  • Incineration
  • Composting
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11
Q

Biosecurity and biosafety

A

-All specimens should be handled and treated as though they are a potential zoonosis threat

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12
Q

Routes of exposure to pathogens during a postmortem

A
  • Oral
  • Splash
  • Aerosolization
  • Percutaneous (sharps injury)
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13
Q

What is the #1 route of exposure of pathogens during a postmortem?

A

Oral

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14
Q

BSL for routine necropsy of a domestic animal

A

2

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15
Q

BSL for animal suspected to have rabies

A

3

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16
Q

BSL for a person with immunosuppression/pregnancy

A

3

17
Q

Equipment needed

A
  • PPE
  • Sharp knife
  • Rib cutters
  • Saw or axe for brain removal
  • Forceps, scissors
  • Jars, bags
  • Swabs, tubes
18
Q

Why is it important to inspect the premise and examine other animals?

A
  • Toxic plants
  • Good to check animals for clinical signs
19
Q

Postmortem steps for ANY species

A
  • External examination
  • Open body cavities
  • Collect microbiologic samples
  • Remove and dissect organs
  • Collect histologic samples
20
Q

Foramlin will not penetrate more than ____ cm tissue.

A

1/2

21
Q

Organs with a capsule need to be ______ in a histologic sample.

A

Incised - cut open!!

22
Q

Formalin:Tissue ration

A

10:1

23
Q

Don’t try to fix tissue that is more than _____ cm in diameter.

A

1

24
Q

After the postmortem

A
  • Clean and disinfect work surfaces
  • Decontaminate self by removing gloves and coveralls, washing boots/vehicle tires/wheel bases
  • Record necropsy findings
25
Q

What body cavity do you open first?

A

Abdominal

26
Q

Once the body cavities are open, which test do you sample for first?

A

Microbiology

27
Q

Collecting Microbiologic samples

A
  • Collected first
  • Collected aseptically
  • Sample intestine last due to fluid
  • Keep samples cold with ice/gel packs
  • Direct sample towards most likely cause
  • Place fresh tissues in separate containers
  • Collect generous tissue samples = swabs
28
Q

Red top/plain sterile tube

A

Culture

29
Q

EDTA/purple top

A
  • Fluid analysis
  • Cytology
30
Q

Removing and dissecting organs

A
  • Open ALL lumens
  • Examine ingesta = foregin material, toxic plants, feed quality, etc.
  • Evaulate mucosal surfaces
31
Q

Collecting Histologic samples

A
  • All organs of the body especially those with lesions
  • Better for lab to discard unneeded samples than for the organ that is needed not be sampled
  • Label tissues if needed
  • Toxins = urine, stomach contents, fat