Autophagy

Question 1

• What is autophagy?
• Microautophagy?
• Chaperone mediated autophagy? Vessicle? AA sequence that is recognized?
• Macroautophagy?
• 3 general steps of macro autophagy?
• Endosome + autophagosome? Add lysome?
• Complex that is made before 3 steps happen?
• Autophagy and cancer therapy?
• When is autophagy induced? (4)
• Autophagy often converges on what pathway?
• What genes regulate it?

Answer 1

• How stuff is delivered to lysosome for degrad.
• Don’t know much
• Chaperone selects proteins for degrad.; no need; KFERQ
• Vessicles necessary to degrade stuff in cell
• Autophagosome forms; adds endosome; lysome binds and recycles material
• Amphisome; Autolysome
• PI3K complex
• Involved in tumor supression and promotion
• Starvation, after birth; Rapamyocin; chemo
• mTOR
• AtG genes

Question 2

• What is often tied with autophagy? BcL2 Example?
• Ex?
• Starvation induced cell death? Stress induced cell death?
• BH3 to Beclin 1 leads to?
• Role of Rapamyacin: 4 steps; protects against?
• Autophagy often degrades what type of proteins?
• May protect against? (3)
• Toxic stimulus? Neuronal cel death?
• Treatments work best in?

Answer 2

• Apoptosis - Regulates cell death and autophagy
• Caspase (apoptotic protease) cleavage essentially blocks autophagy
• Autophagy makes sense; autophagy doesn’t have obvious benefit
• autophagy
• Rapamycin –> mTOR –> atG proteins –> Autophagy; glutamine toxicity
• Aggregate prone
• Alzheimers, Huntingtons, Ataxia
• No; No
• Combination