Automation in transfusion Flashcards
What are the Advances in Automated Blood Grouping and Screening Technology
Scanning
Photography
Programming for workflow
Interfacing
Sharing of images and information
Troubleshooting
What is an example of programming for workflow
Running panels, phenotyping and crossmatching
reflex testing
What is Interfacing
-letting instrument interpret result and upload to LIS system
-operator will verify data and release results
Why are images shared between sites
Assistance with interpretation
Confirmation of reaction strength when referral is required
What is needed for validation
needs to be done before use
-Collect 100 samples to meet criteria
-Normal and Abnormal data needed
-freeze samples or purchase them
-automated method must correlate excellently with manual
-calculate % error
when should you avoid implementing instruments
during the months of December/January or July/August
-ensure there is overlap between old and new systems
Cost
automation reduces cost
-Quantity of test outweighs the cost of the reagents/QC material
benefits for techs with automation
Error Reduction
Sample ID
Anti-Sera and Reagents red cell dispense
Standardization
Unbiased interpretation
Technique consistency
why do red cells need a stir ball
red cells settle and false positive results.
ProVue by Ortho
automated MTS
Tango Biorad
traditional hemagglutination method
microplate wells/strips for Hemagglutination assays
-ABO forward and reverse
-plates coated with monoclonal antisera
-suspension of RBC in bromelin
-incubate at RT
-spin
-shake to resuspend neg reactions
-use with validator
Pos - pellet
Neg - diffusion
Tango Biorad
solid phase method
-Protein A coated microplate
-incubate at 37
centrifuge
positive = diffusion
negative - pellet
