Assay Tests

Question 1

Southern Blot

Answer 1

Probe –> ssDNA
Target –> DNA
Use –> Finding specific genes

Question 2

Northern Blot

Answer 2

Probe –> ssDNA
Target –> mRNA
Use –> Size and quantity of mRNA

Question 3

Western Blot

Answer 3

Probe –> Ab
Target –> Protein
Use –> | HIV Ags | [Ag or Protein] |

Question 4

PCR

Answer 4

Probe –> DNA Primer
Target –> Sequence to be amplified
Use –> | detection of microorganisms | cystic fibrosis |

Question 5

RFLP

Answer 5

Probe –> ssDNA
Target –> DNA
Use –> | Forensics | paternity test |

Question 6

VNTR

Answer 6

Probe –> Restriction Enzymes
Target –> Short tandem Repeats
Use –> | Huntington’s | Fragile X | Friedrich Ataxia |

Question 7

Recombinant Proteins

Answer 7

Probe –> Human DNA
Target –> Plasmid
Use –> | Insulin | Clotting factors | Growth factors

Question 8

Indirect ELISA

Answer 8

Probe –> Ag
Target –> Ab
Use –> ?

Question 9

Sandwich ELISA

Answer 9

Probe –> Ab
Target –> Ag
Use –> | HIV (flourescent or colored) |