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IS LAB SEMIS CRP & ASO > ASO > Flashcards

ASO Flashcards

1
Q

____________– common bacteria responsible for sore throat but most common cause
are the _____________.

A

Streptococci
Viruses

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1
Q

Identify characteristics/ complications of the following:
Scarlet fever –
Rheumatic fever –
Glomerulonephritis –

A

Scarlet fever – red rashes
Rheumatic fever – cardiac issues
Glomerulonephritis – worst kidney problem

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2
Q

No lysis of red blood cells

A

Gamma hemolysis

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3
Q

Incomplete lysis of red blood cells, results in a greenish halo

A

Alpha hemolysis

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4
Q

Complete lysis of red blood cells and a clear zone surrounding the colonies.

A

Beta hemolysis

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5
Q

Differentiate S. pneumoniae & S. viridans

A
  • S. pneumoniae – optochin sensitive and bile soluble
  • S. viridans – optochin resistant and bile insoluble
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6
Q

Gamma-hemolytic bacteria

A

Enterococcus group

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7
Q

Beta-hemolytic bacterias

A
  1. Group A – S. pyogenes
  2. Group B – S. agalactiae
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8
Q

Alpha-hemolytic bacteria

A

S. pneumoniae
S. viridans

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9
Q

differentiate the beta-hemolytic streptococcus group using Bacitracin test

A
  1. Group A – S. pyogenes : Sensitive
  2. Group B – S. agalactiae : Resistant
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10
Q

differentiate the beta-hemolytic streptococcus group using CAMP Test

A
  1. Group A – S. pyogenes : Negative
  2. Group B – S. agalactiae : Positive
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11
Q

Standard method for identifying the
group b streptococcus serotype methods.

A

Lancefield precipitation test

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12
Q

Tests to differentiate the beta-hemolytic streptococcus group:

A

Bacitracin test
CAMP (Christie–Atkins–Munch-Peterson) test
Lancefield precipitation test

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13
Q

hemolytic factor produced by most strains of Group A beta-hemolytic streptococci

A

STREPTOLYSIN O

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14
Q

streptolysin O, the O stands for _______

A

oxygen- labile

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15
Q

streptolysin S, the S stands for _______

A

oxygen-stable

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16
Q

Differentiate Streptolysin O & Streptolysin S

A

Streptolysin O – active only on reduced state. Is antigenic and give rise to ASO antibodies
Streptolysin S – active both on reduced and oxidized condition. Non-antigenic

17
Q

enzyme that causes complete hemolysis on blood agar plate.

A

Streptokinase

17
Q

enzyme that specifies the chromatin breakdown during apoptosis or necrosis.

A

DNAse

18
Q

State the causes of a hemolyzed sample

A

Incorrect needle size
Excessive suction
Improper tube mixing
Prolonged tourniquet application
Improper tube filling
Difficulty in extraction

19
Q

ASO appears in serum from ___________ after the onset of a streptococcal infection

A

1 week – 1 month

20
Q

ASO Normal ranges:
Children:
Adult:

A

Normal ranges:
Children = <150 iu/ml
Adult = <200 iu/ml

21
Q

Incubation period for Streptococcal Infections

A

2nd week – signs & symptoms appears
5th week – peak

22
Q

T or F
Time collection is important to avoid false results

A

T

23
Q

best way to confirm false results

A

Anti-DNAse test

24
Q

use to determine the organisms ability to
hydrolyze the DNA and use it as carbon and energy for growth.

A

DNAse test

25
Q

T or F
Incidence decreases as the time increases

A

T

26
Q

antibody levels begin to rise after________

A

1 to 3 weeks

27
Q

antibody levels begin to peak _____

A

3 to 5 weeks

28
Q

antibody levels falls back to insignificant levels in _____

A

6 months

29
Q

If the titer doesn’t decrease with time; this means that a ________

A

recurrent or chronic infection may exist.

30
Q

Anti-streptolysin O can react specifically with _____ and inhibits the hemolytic activity

A

SLO

31
Q

T or F
The amount of ASO can be estimated by dilution of patient’s serum in the
presence of constant amount of SLO to the point where there is no prevention of hemolysis.

A

F
there is still complete prevention of hemolysis.

32
Q

COMMERCIALLY AVAILABLE TESTS

Used for screening a significant raise in ASO titer

A

ASO LATEX SLIDE TEST

33
Q

COMMERCIALLY AVAILABLE TESTS

Used to determine the titer of ASO antibody

A

ASO TITRATION TEST

34
Q

Principle of RAPID ASO LATEX AGGLUTINATION TEST

A

In the presence of ASO antibody, a visible agglutination reaction will be
exhibited when a serum specimen combine with latex particle coated with
streptolysin O antigen.

35
Q

Specimen for RAPID ASO LATEX AGGLUTINATION TEST

A

serum that is clear and hemolysis free

36
Q

REAGENT STORAGE AND STABILITY

A

Reagent are stable until stated expiration date.
Must be refrigerated (2-8C)
DO NOT FREEZE
ASO Latex reagent, once shaken must be uniform without visible clumping.
Do not use the latex reagent or controls if become contaminated.

37
Q

If the test cannot be carried out on the same day, store the specimen for
7 days at _______. If it will not be used for 3 months or more, store at ______.

A

2-8 C
-20 C

38
Q

T or F
we can use plasma in testing for ASO

A

F
Serum only

IS LAB SEMIS CRP & ASO (2 decks)

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