ASO

Question 1

____________– common bacteria responsible for sore throat but most common cause
are the _____________.

Answer 1

Streptococci
Viruses

Question 1

Identify characteristics/ complications of the following:
Scarlet fever –
Rheumatic fever –
Glomerulonephritis –

Answer 1

Scarlet fever – red rashes
Rheumatic fever – cardiac issues
Glomerulonephritis – worst kidney problem

Question 2

No lysis of red blood cells

Answer 2

Gamma hemolysis

Question 3

Incomplete lysis of red blood cells, results in a greenish halo

Answer 3

Alpha hemolysis

Question 4

Complete lysis of red blood cells and a clear zone surrounding the colonies.

Answer 4

Beta hemolysis

Question 5

Differentiate S. pneumoniae & S. viridans

Answer 5

• S. pneumoniae – optochin sensitive and bile soluble
• S. viridans – optochin resistant and bile insoluble

Question 6

Gamma-hemolytic bacteria

Answer 6

Enterococcus group

Question 7

Beta-hemolytic bacterias

Answer 7

1. Group A – S. pyogenes
2. Group B – S. agalactiae

Question 8

Alpha-hemolytic bacteria

Answer 8

S. pneumoniae
S. viridans

Question 9

differentiate the beta-hemolytic streptococcus group using Bacitracin test

Answer 9

1. Group A – S. pyogenes : Sensitive
2. Group B – S. agalactiae : Resistant

Question 10

differentiate the beta-hemolytic streptococcus group using CAMP Test

Answer 10

1. Group A – S. pyogenes : Negative
2. Group B – S. agalactiae : Positive

Question 11

Standard method for identifying the
group b streptococcus serotype methods.

Answer 11

Lancefield precipitation test

Question 12

Tests to differentiate the beta-hemolytic streptococcus group:

Answer 12

Bacitracin test
CAMP (Christie–Atkins–Munch-Peterson) test
Lancefield precipitation test

Question 13

hemolytic factor produced by most strains of Group A beta-hemolytic streptococci

Answer 13

STREPTOLYSIN O

Question 14

streptolysin O, the O stands for _______

Answer 14

oxygen- labile

Question 15

streptolysin S, the S stands for _______

Answer 15

oxygen-stable

Question 16

Differentiate Streptolysin O & Streptolysin S

Answer 16

Streptolysin O – active only on reduced state. Is antigenic and give rise to ASO antibodies
Streptolysin S – active both on reduced and oxidized condition. Non-antigenic

Question 17

enzyme that causes complete hemolysis on blood agar plate.

Answer 17

Streptokinase

Question 17

enzyme that specifies the chromatin breakdown during apoptosis or necrosis.

Answer 17

DNAse

Question 18

State the causes of a hemolyzed sample

Answer 18

Incorrect needle size
Excessive suction
Improper tube mixing
Prolonged tourniquet application
Improper tube filling
Difficulty in extraction

Question 19

ASO appears in serum from ___________ after the onset of a streptococcal infection

Answer 19

1 week – 1 month

Question 20

ASO Normal ranges:
Children:
Adult:

Answer 20

Normal ranges:
Children = <150 iu/ml
Adult = <200 iu/ml

Question 21

Incubation period for Streptococcal Infections

Answer 21

2nd week – signs & symptoms appears
5th week – peak

Question 22

T or F
Time collection is important to avoid false results

Answer 22

T

Question 23

best way to confirm false results

Answer 23

Anti-DNAse test

Question 24

use to determine the organisms ability to hydrolyze the DNA and use it as carbon and energy for growth.

Answer 24

DNAse test

Question 25

T or F Incidence decreases as the time increases

Answer 25

T

Question 26

antibody levels begin to rise after________

Answer 26

1 to 3 weeks

Question 27

antibody levels begin to peak _____

Answer 27

3 to 5 weeks

Question 28

antibody levels falls back to insignificant levels in _____

Answer 28

6 months

Question 29

If the titer doesn’t decrease with time; this means that a ________

Answer 29

recurrent or chronic infection may exist.

Question 30

Anti-streptolysin O can react specifically with _____ and inhibits the hemolytic activity

Answer 30

SLO

Question 31

T or F The amount of ASO can be estimated by dilution of patient’s serum in the presence of constant amount of SLO to the point where there is no prevention of hemolysis.

Answer 31

F there is still complete prevention of hemolysis.

Question 32

COMMERCIALLY AVAILABLE TESTS Used for screening a significant raise in ASO titer

Answer 32

ASO LATEX SLIDE TEST

Question 33

COMMERCIALLY AVAILABLE TESTS Used to determine the titer of ASO antibody

Answer 33

ASO TITRATION TEST

Question 34

Principle of RAPID ASO LATEX AGGLUTINATION TEST

Answer 34

In the presence of ASO antibody, a visible agglutination reaction will be exhibited when a serum specimen combine with latex particle coated with streptolysin O antigen.

Question 35

Specimen for RAPID ASO LATEX AGGLUTINATION TEST

Answer 35

serum that is clear and hemolysis free

Question 36

REAGENT STORAGE AND STABILITY

Answer 36

Reagent are stable until stated expiration date. Must be refrigerated (2-8C) DO NOT FREEZE ASO Latex reagent, once shaken must be uniform without visible clumping. Do not use the latex reagent or controls if become contaminated.

Question 37

If the test cannot be carried out on the same day, store the specimen for 7 days at _______. If it will not be used for 3 months or more, store at ______.

Answer 37

2-8 C -20 C

Question 38

T or F we can use plasma in testing for ASO

Answer 38

F Serum only