Aseptic Techniques Flashcards
____ are a set of routine measures taken to prevent cultures, sterile media stocks, and other solutions from being contaminated.
Aseptic Techniques
In the laboratory, you must wear appropriate Lab PPE such as the ff: (6)
- long hair tied back
- appropriate hand protection
- closed-toe shoes
- protective eyewear
- buttoned-up, fitted coat
- full-length pants
What are the different biosafety levels (BSL)? (4)
- BSL-1 - not known to cause disease in adult humans.
- BSL-2 - moderate danger if inhaled, swallowed, or exposed to skin.
- BSL-3 - severe or potentially lethal disease.
- BSL-4 - life-threatening disease.
What are some sample organisms for BSL-1?
- E. coli K-12
- Saccharomyces cerevisiae, and
- Non-infectious Bacteria
What are some sample organisms for BSL-2?
- Hepatitis A Virus
- Streptococcus pyogenes
- Borrelia burgdorferi (Lyme Disease)
- Salmonella spp.
What are some sample organisms for BSL-3?
- Yersinia pestis (Plague)
- Mycobacterium tuberculosis
- SARS
- Rabies Virus
- West Nile Virus
- Hantaviruses
What are some sample organisms for BSL-4?
- Ebola Virus
- Smallpox Virus
BSL: not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment.
BSL-1
BSL: a moderate potential hazard to personnel and the environment. Includes bacteria and viruses that cause mild disease to humans, or are difficult to contract via aerosol in a lab setting.
BSL-2
BSL: microbes there can either indigenous or exotic, and they can cause serious or potentially lethal disease through respiratory transmission.
BSL-3
BSL: dangerous and exotic, posing a high risk of aerosol-transmitted infections. Infections caused by these microbes are frequently fatal and without treatment or vaccines.
BSL-4
How to properly prepare a cultural medium? (5)
- weigh dehydrated media or media ingredients
- dissolve components in a solvent
- check or adjust the pH
- cap or plug with a cotton
- sterilize (at 15 psi for 15 minutes)
