asdf2 Flashcards
1
Q
PCR: function of MgCl2
A
stabilizes the DNA backbone
is a cofactor for DNA polymerase (as well as lots of other enzymes)
2
Q
define cofactor
A
a substance (other than the substrate) whose presence is essential for the activity of an enzyme.
3
Q
what’s the point of the buffer in PCR?
A
to moderate the pH of the reaction
