Appearance Flashcards
Buttery looking
Pinhead colonies
Staphylococcus aureus
Gray, pinpoint colonies
Staphylococcus epidermidis
Oval/lancet-shaped
“dimple-shaped” “dome-shaped”
Streptococcus pneumoniae
Gram-variable, pleomorphic
“Satellites” around organism with pyridoxal
Abitrophia, Granulicatella
Coffee / kidney-bean shaped
Neisseria gonorrhoeae
Rod- shaped
“N. WEB”
Neisseria elongata
Neisseria bacilliformis
Neisseria weaver
Bluish gray colonies on BAP
Neisseria meningitidis
“Bread-crumbs”
Neisseria sica
Resemble Neisseria; grow from end to end with adjacent sides flattened
“Hockey-puck” appearance
Moraxella catarrhalis (Branhamella catarrhalis)
“Stacked brick” appearance
Enteroadherent E. Coli (EAEC)
Musty, pungent, “rotten potato-like” odor
Serratia odorifera
“Burnt chocolate or gunpowder” odor
Proteus
Closed safety pin, with bipolar bodies
Broth: Stalactite-shaped pattern
Yersinia pestis
With bipolar bodies
“Bull’s eye” appearance in CIN
Yersinia enterolitica
Comma, curved bacilli
Vibrio
“Bull’s eye” appearance in CIN
Aeromonas
S-shaped rods, seagull wing-shaped
Tailing effect, spreading
Campylobacter
S-shaped rods, similar to Campylobacter
Helicobacter
Metallic sheen in BAP, “grape-like” or “corn tortilla” odor
Pseudomonas aeruginosa
Mistaken as Neisseria
Acinetobacter
“Ammonia” odor
BAP = lavender-green colonies
MAC = blue colonies
Stenotrophomonas maltophilia
Non-wrinkled yellow colonies in BAP
Buckholderia cepacia
With bipolar bodies
Buckholderia pseudomallei
Comma, curved bacilli
Violet (violacein pigment)
Chromobacterium violaceum
Fruity odor (“apples or strawberries”)
Alcaligenes faecalis
Mucoid and greenish (including media)
Shewanella putrefaciens
“Amorphous serous material”
Haemophilus spp.
“Mousy” or “bleach-like” odor
Haemophilus influenzae
“School of fish”
Haemophilus ducreyi
“Star-shaped” appearance
Aggregibacter actinomycetemcomitans
“False Gram positive” reactions in some parts of cells
Exhibit “rosette” formation, pits agar
Cardiobacterium hominis
“Sandy” appearance
Brucella
“Mercury drops” in Berdet-Gengou agar
Bordetella pertussis
Faint bipolar staining
Francisella tularensis
“Mushroom smell”
Pasteurella multocida
“Rainbow color” colonies in buffered charcoal yeast extract (BCYE) agar
Legionella
Box-shaped with “empty spaces” (central spores)
Bacillus spp.
“Bamboo fishing rod” appearance
BAP = “Medusa head” with swirling projection
Inoculating loop = “beaten egg white” appearance
Penicillin = “string of pearls”
Gelatin medium = “inverted pine tree”
Bacillus anthracis
“Frosted glass” appearance; spreading growth
Bacillus cereus
“Ground glass” appearance; exhibit pigment atoms in BAP
Bacillus subtilis
“Blister-like” appearance in BAP
Bacillus pumilus
“Club-shaped” swellings Highly pleomorphic, resemble Chinese letters “Poached egg” Biotype mitis = “Fried egg” Biotype gravis = “Daisy head”
Corynebacterium diptheriae
V-shaped forms and palisades
Pinpoint colonies
Corynebacterium urealyticum
Parallel rows or palisades; does not exhibit any other characteristic “pleomorphism” from other spp.
Corynebacterium pseudodiptheriticum
Club-shaped, V forms
Corynebacterium jeikeium
Resemble Streptococci spp.
Listeria monocytogenes
“Pipe cleaner” or “test tube brush” appearance in gelatin stab culture
Erysipelothrix rhusiopathiae
BAP = “Medusa head”
NA = Rhizoid growth
Kurthia
Beaded appearance
Aerobic Actinomycetes
Wrinkled, chalk-like, orange-tan pigmentation
Nocardia
Very similar to Nocardia spp.
“Molar tooth” appearance 
Actinomadura
“Musty basement” odor
Streptomyces
“Zigzag pattern”
Rhodococcus equi
“Sharp bleach odor” pits agar
Eikenella corodens
“Cauliflowerxlike” with coroding
Mycobacterium tuberculosis
Distinct crossbanding
Culture: exhibit dark red crystals of 10-b-carotene
Mycobacterium kansaii
With cross-barring
Mycobacterium marinum
No crossbanding
Mycobacterium ulcerans
“Cigar-shaped” or “pocket-fence” arrangement
Mycobacterium leprae
Subterminal spores “boxcar-shaped”
BAP: double zone of hemolysis
Litmus-milk: Stormy fermentation
Clostridium perfringes
Terminal spores, “drumstick” or “tennis racket” appearance
Clostridium tetani
Subterminal spores
Clostridium botulinum
Subterminal spores
Culture: “ground-glass” appearance in CFFA
BAP: horse-stable odor
Clostridium difficile
Young colonies: “Spider-like” or “wooly”
Old colonies: “Molar tooth”
Anaerobic Actinomycetes
“Dog bone” shape. Pits agar
Bacteroides ureolyticus
“Seagull wing” shaped; fluorescent chartreuse color
Eubacterium
Media turns green upon air exposure, “breadcrumb-like” colonies
Fusobacterium
“Mulberries” or morula
Ehrlichia
“Fried egg appearance”
Mycoplasma hominis
Dark brownish lumps on A7 or A8 agar
Ureaplasma urealyticum
“L forms or “yeast-like” shape
BAP - “Fried egg” appearance
Broth - “fluff balls/ bread crumps”
Streptobacillus moniliformis
Safety pin appearance, presence of donovan bodies
Klebsiella (calymmatobacterium) granulomatis
Spindle-shaped (slight yellow or orange pigmentation)
Capnocytophaga